The use of growth factors in the proliferation of avian articular chondrocytes in a serum-free culture system

Connective Tissue Research
K T RouscheRoland M Leach

Abstract

The purpose of this research was to develop a serum-free culture system for the proliferation of articular chondrocytes. Various growth factors and hormones were tested for their ability to stimulate avian articular chondrocyte proliferation in a defined, serum-free media. Multiple members of the fibroblast growth factor (FGF) family (FGFs: 2, 4, and 9), insulin-like growth factor-1 (IGF-1) and transforming growth factor beta (TGF-beta) significantly stimulated H-thymidine uptake by chondrocytes grown in an adherent serum-free, culture system. Double or triple combinations of these mitogenic growth factors further stimulated cell proliferation to levels that were equivalent to, or surpassed those of cells grown in serum. Although proliferation was maximally stimulated, chondrocytes grown in the presence of FGF-2, IGF-1, and TGF-beta, began to exhibit changes in morphology and collagen II expression declined. This culture system could be used to rapidly expand a population of articular chondrocytes prior to transferring these cells to a non-adherent culture system, which could then stabilize the chondrocyte phenotype and maximize matrix synthesis and integrity.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H LehrachP Doty
Jan 1, 1992·Calcified Tissue International·I D CrabbR N Rosier
Apr 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G RosselotR M Leach
Aug 1, 1991·Archives of Biochemistry and Biophysics·T I MoralesA B Roberts
Jun 1, 1989·Current Opinion in Immunology·R Kath, M Herlyn
Jan 1, 1989·Annual Review of Genetics·L M AlbrightF M Ausubel
May 25, 1984·Nucleic Acids Research·M F YoungM E Sobel
May 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·T Barak-ShalomM Pines
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Sep 1, 1995·Calcified Tissue International·T TsukazakiS Yamashita
Mar 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J ShidaY Sugioka
Feb 1, 1997·Anatomy and Embryology·C Grothe, C Meisinger
Sep 28, 1998·General and Comparative Endocrinology·T ShimadaH Nishimura
Jan 1, 2000·The Journal of Bone and Joint Surgery. British Volume·J B RichardsonS Roberts

❮ Previous
Next ❯

Citations

Dec 28, 2006·Cells, Tissues, Organs·Paolo Giannoni, Ranieri Cancedda
Jan 12, 2002·Journal of Cellular Biochemistry·Craig A PraulRoland M Leach
Feb 3, 2004·The American Journal of Sports Medicine·Constance R ChuRebecca K Studer
Jun 4, 2008·Annals of Plastic Surgery·Noritaka IsogaiHiroshi Munakata
Mar 20, 2012·Journal of Abnormal Child Psychology·K MonshouwerW A M Vollebergh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.