The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response

Memórias do Instituto Oswaldo Cruz
Daiane Drawanz HartwigOdir Antônio Dellagostin

Abstract

We studied the feasibility of using halloysite clay nanotubes (HNTs) and carboxyl-functionalised multi-walled carbon nanotubes (COOH-MWCNTs) as antigen carriers to improve immune responses against a recombinant LipL32 protein (rLipL32). Immunisation using the HNTs or COOH-MWCNTs significantly increased the rLipL32-specific IgG antibody titres (p < 0.05) of Golden Syrian hamsters. None of the vaccines tested conferred protection against a challenge using a virulent Leptospira interrogans strain. These results demonstrated that nanotubes can be used as antigen carriers for delivery in hosts and the induction of a humoral immune response against purified leptospiral antigens used in subunit vaccine preparations.

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Citations

Apr 23, 2020·Memórias do Instituto Oswaldo Cruz·Thaís Larré OliveiraDaiane D Hartwig
Apr 10, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Junxia DuanFeijun Zhao
Jun 3, 2021·Materials·Anna KarewiczGabriela Ledwójcik

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Methods Mentioned

BETA
Protein Assay
X-ray
electrophoresis
ELISA

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