The use of human and bovine commercial media for oocyte maturation and embryo development in the domestic cat (Felis catus)

Reproduction in Domestic Animals = Zuchthygiene
S ProchowskaMarcin Pałys

Abstract

The aim of this study was to examine the suitability of commercial media designed for humans and cattle for oocyte maturation and embryo culture in the domestic cat. In Exp. I, feline oocytes collected ex vivo were subjected to in vitro maturation in a laboratory-made culture medium (based on M199) or a commercial medium designed for cattle cells (BO-IVM® ). In Exp. II, ICSI-derived feline embryos were cultured for 7 days in a commercial human (Continuous Single Culture® ) or bovine (BO-EC® ) cell medium. The rates of cleavage, morula and blastocyst formation were evaluated at 24 hr, 6 days and 7 days after ICSI, respectively, and compared between experimental groups. At the end of culture, embryos were assessed for viability and apoptotic changes. In Exp. I, no statistically significant difference in oocyte maturation outcome between laboratory-made (52.7%) and commercial media (58.9%) was observed. However, the use of a commercial medium prepared for use with bovine cells resulted in a significantly lower variance of the maturation rate. In Exp. II, no statistically significant differences between two commercial media were observed for cleavage (67.5% and 64.5%), morula (39.3% and 47.1%) and blastocyst rates (25.0% and 19.6%)...Continue Reading

References

Dec 1, 1970·Journal of Reproduction and Fertility·C E HamnerN J Sojka
Jun 1, 1993·The Japanese Journal of Psychiatry and Neurology·S Ohtahara, M Seino
Sep 8, 2001·Reproduction, Fertility, and Development·M C GómezB L Dresser
Dec 7, 2002·Reproduction, Fertility, and Development·N W Kurniani KarjaTatsuyuki Suzuki
Apr 13, 2005·Theriogenology·Barbara MerloStefano Belluzzi
May 3, 2007·Molecular Reproduction and Development·Raúl Fernández-GonzalezAlfonso Gutiérrez-Adán
Jul 28, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·R WaurichK Jewgenow
Jun 28, 2012·Reproduction in Domestic Animals = Zuchthygiene·R HribalP Comizzoli
Jul 19, 2014·Human Reproduction Update·Elpiniki Chronopoulou, Joyce C Harper
Aug 25, 2016·Human Reproduction·Arne SundeAnna Veiga
May 20, 2017·Polish Journal of Veterinary Sciences·S Prochowska, W Niżański
Nov 27, 2018·Reproduction in Domestic Animals = Zuchthygiene·Gaia Cecilia LuvoniMaria Giorgia Morselli

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Citations

Aug 8, 2021·Animals : an Open Access Journal From MDPI·Martina ColomboGaia Cecilia Luvoni

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