The use of in-situ normothermic extracorporeal perfusion and leukocyte depletion for resuscitation of human donor kidneys

Perfusion
O ReznikY Moysyuk

Abstract

The unexploited potential of donors after cardiac death is an actual issue for all countries where organ transplantation is performed. The crucial point in uncontrolled donation after cardiac death is the warm ischemic time. The primary purpose of our work was to define the limits of warm ischemic time. Another purpose was the development of an in situ kidney preservation protocol. In 8 uncontrolled donors with warm ischemic time from 45 to 91 minutes, a normothermic extracorporeal perfusion device was applied, providing preservation and restoration of the kidney after ischemic damage. Main attention was paid to the elimination of leukocytes as the key damaging factor from modified donor blood circulating in the device. In 6 out of 16 kidney recipients, graft function was recovered immediately and, by the end of the third month, the average creatinine was 117.9±21.9 mmol/L. Treatment of ischemically damaged kidneys by normothermic extracorporeal perfusion, with leukocyte depletion before procurement, seems to be a challenging protocol and demands further study. Implementation of perfusion systems in organ procurement practice could lead to a partial solution in the organ deficit problem.

References

Jan 9, 1998·Transplantation·J F BurdickL E Bennett
Jan 13, 2001·Clinical Chemistry and Laboratory Medicine : CCLM·S BalupuriD Talbot
Jul 9, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·D Shoskes
Jul 19, 2003·Perfusion·Piya SamankatiwatTerence Gourlay
Sep 27, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·B MirandaJ M Grinyó
Mar 3, 2004·The Annals of Thoracic Surgery·George M PalatianosGeorge M Astras
Jul 5, 2005·The Journal of Trauma·Joseph F MaglioccaMark R Hemmila
Jan 24, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J L BernatF L Delmonico
Jan 24, 2006·The Annals of Thoracic Surgery·Per WierupStig Steen
May 12, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L MatsuokaR Selby
Aug 2, 2006·Annals of Internal Medicine·Ana I Sánchez-FructuosoAlberto Barrientos
Feb 27, 2007·Nature Clinical Practice. Nephrology·Gauke Kootstra, Ernest van Heurn
May 29, 2007·The Annals of Thoracic Surgery·Stig SteenTrygve Sjöberg
Jun 15, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C FondevilaJ C García-Valdecasas
Aug 19, 2007·The British Journal of Surgery·A BagulM L Nicholson
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Russell W Jamieson, Peter J Friend
Dec 9, 2008·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Marcelo CypelShaf Keshavjee
Dec 23, 2008·The Annals of Thoracic Surgery·Richard IngemanssonStig Steen
Mar 19, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M FiguraL A Tibbles
Mar 20, 2009·Transplantation·Srikanth P ReddyPeter J Friend
Aug 12, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M CypelS Keshavjee
Oct 15, 2009·Critical Care : the Official Journal of the Critical Care Forum·Bradley J KaufmanUNKNOWN New York City Uncontrolled Donation after Cardiac Death Study Group

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Citations

Mar 19, 2011·Current Opinion in Organ Transplantation·Sarah A Hosgood, Michael L Nicholson
Sep 28, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Julie De BeuleIna Jochmans

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