The use of insects as a bioassay for Penaeus merguiensis densovirus (PmergDNV)

Journal of Invertebrate Pathology
Kathy A La Fauce, Leigh Owens

Abstract

The lack of available cell lines has hampered the study of viral diseases in crustaceans. This is particularly important for aquaculture which has been plagued by viral diseases since its rapid expansion to meet with the growing demand for seafood products. This study was designed to find an alternative bioassay to cell lines by investigating the use of insects as potential animal models for Penaeus merguiensis densovirus (PmergDNV). Acheta domesticus (house cricket) and Tenebrio molitor (mealworms) were challenged with approximately 1x10(6) virions of PmergDNV by inoculation. PmergDNV was detected in 20% of Tenebrio molitor and 86.6% of Acheta domesticus challenged with PmergDNV. During a subsequent time course experiment, there was a non significant increase in PmergDNV titres (10(4-5) virions), reaching a maximum peak at day 5 (10(6) copies). A threshold of PmergDNV DNA level equal to or greater than 10(3) virions was necessary for mortality in Acheta domesticus. As the inoculum increased from 10(3) DNA copies to 10(4), 10(5), 10(6), mortality increased from 20% to 60%, 80% and 100%, respectively. This is the first evidence that insects may be directly used to study viruses from crustaceans and concludes Acheta domesticus ma...Continue Reading

References

Sep 16, 2004·Journal of Virology·Javier RobalinoGregory Warr
Jan 25, 2005·Emerging Infectious Diseases·Carlos R PantojaCharles P Gerba
Oct 3, 2006·Antiviral Research·Jianyang XuXiaobo Zhang
Nov 23, 2006·Journal of Virological Methods·Kathy A La FauceLeigh Owens
Mar 28, 2007·Journal of Medical Virology·J GooskensA C Kroes

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Citations

Sep 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·Muhammed P SafeenaIndrani Karunasagar
Sep 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·Kathy La Fauce, Leigh Owens
Nov 26, 2008·Journal of Invertebrate Pathology·Kathy A La Fauce, Leigh Owens
May 31, 2019·Virusdisease·Arun K DharDarryl Jory
Aug 26, 2021·EFSA Journal·UNKNOWN EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA)Helle Katrine Knutsen

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