PMID: 58995May 1, 1976

The use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies

The Journal of Membrane Biology
B SarkadiG Gárdos

Abstract

Techniques are described for the rapid loading of intact human red cells with radioactive isotopes of alkali cations or Ca2+ by using ionophorous compounds (nigericin, gramicidin D and A 23187). Loading was rapid and efficient if the membrane potential of the cells was rendered more negative inside. After cation loading the ionophores could be bound to albumin and removed by repeated washings. The ATP and 2,3-DPG contents of the cells were practically unaltered by this treatment. Passive membrane permeability to Na+ and Ca2+ returned to normal. Loaded erythrocytes pumped out Na+ in a ouabain-sensitive and Ca2+ in a lanthanum-sensitive way. Ca2+ -loaded red cells were microspherocytes and exhibited a rapid K+ -efflux. Parallel with the extrusion of Ca2+ cells regained their biconcave shape and normal passive permeability to K+.

References

Apr 20, 1979·The Journal of Membrane Biology·B SarkadiG Gárdos
Apr 1, 1969·The Journal of Physiology·H J Schatzmann, F F Vincenzi
May 1, 1969·The Journal of Physiology·H Lubowitz, R Whittam
Dec 15, 1973·Molecular and Cellular Biochemistry·G Schwoch, H Passow
Mar 1, 1969·The Journal of General Physiology·E J Olson, R J Cazort
Mar 1, 1969·Acta Physiologica Scandinavica·A EkmanS Salminen
Jul 11, 1973·Nature: New Biology·A Cass, M Dalmark
Dec 1, 1968·The Journal of Physiology·D G Harrison, C Long
Dec 1, 1969·The Journal of General Physiology·K S Lee, B C Shin
May 1, 1971·The Journal of General Physiology·Robert B Gunn, D C Tosteson
Jun 15, 1966·Experientia·H J Schatzmann
Sep 1, 1967·The Journal of Physiology·P J Garrahan, I M Glynn
Nov 1, 1967·The Journal of Physiology·P J Garrahan, A F Rega
Sep 1, 1953·The Journal of Physiology·E J HARRIS, T A PRANKERD
Nov 28, 1956·The Journal of Physiology·I M Glynn
Oct 1, 1965·The Biochemical Journal·R Whittam, M E Ager

Citations

Apr 1, 1992·Journal of Clinical Pharmacology·N W Seidler, N I Swislocki
Mar 1, 1994·Molecular Reproduction and Development·N Tanphaichitr, C Hansen
Jan 31, 1978·Pflügers Archiv : European journal of physiology·H Rogausch
Jun 24, 1977·The Journal of Membrane Biology·I SzászG Gárdos
Aug 4, 1977·The Journal of Membrane Biology·G A Plishker, H J Gitelman
Jan 1, 1984·The Journal of Membrane Biology·E M BifanoJ C Freedman
Jul 18, 1978·The Journal of Membrane Biology·F L LarsenF F Vincenzi
Dec 8, 1978·The Journal of Membrane Biology·J P RossiA F Rega
Aug 1, 1984·Biological Trace Element Research·A Egyed', P Saltman
Jul 25, 1993·Biochimica Et Biophysica Acta·H T TruongW H Huestis
Feb 1, 1994·Pharmacology, Biochemistry, and Behavior·A K Mehta, M K Ticku
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·K Tanabe, S Doi
May 1, 1980·The Journal of Cell Biology·V Fowler, D L Taylor
Jun 1, 1982·The Journal of Cell Biology·K TanabeD F Wallach
Aug 1, 1978·British Journal of Haematology·I SzászG Gárdos
Apr 28, 1978·Annals of the New York Academy of Sciences·H J Schatzmann, H Bürgin
Jan 1, 1986·Free Radical Research Communications·A De FloraL Guida
Dec 11, 1987·Biochimica Et Biophysica Acta·R O Iglesias, A F Rega
Apr 15, 1979·Analytical Biochemistry·M R Mauk, R C Gamble
Mar 20, 1981·Biochimica Et Biophysica Acta·S K Jain, S B Shohet
Apr 1, 1989·Biochemical Medicine and Metabolic Biology·E FriederichsW Tillmann
Dec 1, 1984·European Journal of Clinical Investigation·S F Hoare, C R Paterson
Dec 1, 1984·Journal of Cellular Physiology·A M Benjamin, D M Quastel
Jun 13, 1979·Biochimica Et Biophysica Acta·H Sze, A K Solomon
Nov 22, 1982·Biochimica Et Biophysica Acta·L O SimonsenVirgilio L Lew
May 23, 1976·Biochemical and Biophysical Research Communications·D TaylorP Hochstein
Sep 22, 1978·Biochimica Et Biophysica Acta·I SzászG Gárdos
Jun 27, 1984·Biochimica Et Biophysica Acta·M Nikinmaa, W H Huestis
May 7, 1982·Biochimica Et Biophysica Acta·J E Ferrell, W H Huestis
Apr 10, 1980·Nature·Robert M Bookchin, Virgilio L Lew
Nov 1, 1981·Scandinavian Journal of Clinical and Laboratory Investigation·M Dalmark
May 2, 1983·FEBS Letters·G A Vidaver, J W Lee
Dec 25, 2004·Physiological Reviews·Virgilio L Lew, Robert M Bookchin
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·W A Shannon, D M Zellmer
Nov 1, 1987·The Journal of Experimental Zoology·S S SuarezM W Ceglia
Apr 4, 2003·American Journal of Physiology. Cell Physiology·Sammy Elmariah, Robert B Gunn
Jan 1, 1986·The American Journal of Physiology·J S Adorante, R I Macey
Mar 1, 1990·The American Journal of Physiology·M A Milanick
May 1, 1981·The American Journal of Physiology·E L WatsonW Farnham

Related Concepts

Calcimycin
Adenosine Triphosphate, Chromium Ammonium Salt
Calcium
Chloretone
Diphosphoglyceric Acids
Erythrocytes
Gramicidin NF
Ionophores
Microscopy, Phase-Contrast
Pandavir

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.