PMID: 11933221Apr 5, 2002Paper

The use of low-molecular-weight PEIs as gene carriers in the monkey fibroblastoma and rabbit smooth muscle cell cultures

The Journal of Gene Medicine
Pasi LampelaAtso Raasmaja

Abstract

Polyethylenimines (PEIs) and cationic polymers have been used successfully in gene delivery. In earlier reports, only large PEIs (MW>10 000) have shown significant transfection efficiency. In the present study, the roles of small PEIs (MW 700 and 2000) were studied as additional compounds to see if they can improve gene delivery with cationic liposomes. The TKBPVlacZ expression plasmid was transfected in the CV1-P (monkey fibroblastoma) and SMC (rabbit smooth muscle) cell lines using various combinations of PEIs (MW 700, 2000, and 25 000) and Dosper liposomes. The transfection efficiency was determined with the fluorometric ONPG (o-nitrophenol-beta-D-galactopyranoside) assay and histochemical X-gal staining. The toxicity of the transfection reagents was estimated by the MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide] assay. Transfection of TKBPVlacZ plasmid by the small PEIs (MW 700 and 2000) combined with Dosper liposomes was associated with high expression of the lacZ reporter gene in the CV1-P and SMC cell lines. The transfection efficiencies of the low-molecular-weight PEI/liposome combinations were several fold higher than those of PEIs or liposomes alone. PEI/liposome combinations had no toxicity on the...Continue Reading

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Citations

Jun 15, 2007·Pharmaceutical Research·Enrico MastrobattistaRaymond M Schiffelers
Jan 17, 2004·International Journal of Pharmaceutics·Pasi LampelaAtso Raasmaja
Jun 14, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tae Hee KimChong Su Cho
Feb 15, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pasi LampelaAtso Raasmaja
Jun 5, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sárka MañákováPekka T Männistö
Oct 7, 2010·Journal of Microencapsulation·Conchita Tros de IlarduyaNejat Düzgünes
Mar 13, 2009·Molecular Membrane Biology·Nuno PenachoMaria C Pedroso de Lima
Aug 5, 2003·The Journal of Gene Medicine·Yoon Jeong ParkVictor C Yang
Nov 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangZongshi Li
Nov 22, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mika ReinisaloPaavo Honkakoski
Mar 28, 2003·Biochimica Et Biophysica Acta·Chien-Hsin LeeFu-Hsiung Chang
Sep 11, 2007·Biological & Pharmaceutical Bulletin·Yoshiyuki Hattori, Yoshie Maitani
Oct 24, 2009·Bioconjugate Chemistry·Tony Le GallJean-Luc Décout

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