The use of Matrix-assisted laser desorption ionization-time of flight mass spectrometry in the identification of Francisella tularensis

Bosnian Journal of Basic Medical Sciences
Onur KaratunaSelcuk Kilic

Abstract

Francisella tularensis is the cause of the zoonotic disease tularemia and is classified among highly pathogenic bacteria (HPB) due to its low infection dose and potential for airborne transmission. In the case of HBP, there is a pressing need for rapid, accurate and reliable identification. Phenotypic identification of Francisella species is inappropriate for clinical microbiology laboratories because it is time-consuming, hazardous and subject to variable interpretation. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was recently evaluated as a useful tool for the rapid identification of a variety of microorganisms. In this study, we evaluated the use of MALDI-TOF MS for the rapid identification of Francisella tularensis and differentiation of its subspecies. Using national collection of Francisella isolates from the National Tularemia Reference Laboratory (Public Health Institute of Turkey, Ankara), a total of 75 clinical isolates were investigated by species and subspecies-specific polymerase chain reaction (PCR) test and MALDI-TOF MS. All isolates were originally identified as F. tularensis subsp. holarctica due to RD1 subspecies-specific PCR result. For all isolates MALDI-TOF MS...Continue Reading

Citations

Sep 30, 2020·Folia Microbiologica·Derya Karataş YeniM Salah Ud Din Shah
Jun 13, 2017·The Journal of Antimicrobial Chemotherapy·Herbert TomasoMats Forsman

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Methods Mentioned

BETA
nucleic acid amplification
PCR
electrophoresis

Software Mentioned

Maldi Biotyper
Bruker MALDI Biotyper

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