PMID: 8390473May 1, 1993

The use of MDCK, MEK and LLC-MK2 cell lines with enzyme immunoassay for the isolation of influenza and parainfluenza viruses from clinical specimens

Journal of Virological Methods
S Schepetiuk, T W Kok

Abstract

Primary Monkey Kidney (PMK) epithelial cells or egg inoculation have been traditionally used for the culture of influenza and parainfluenza viruses. The high cost and variability of obtaining high quality PMK cells prompted us to investigate the use of other cell strains for the growth of these viruses. For this study we investigated three cell lines viz. MDCK, MEK and LLC-MK2 for the culture of influenza A and B and parainfluenza 1, 2 and 3 viruses. Clinical specimens were spun onto cell monolayers in microtitre wells. The growth of these viruses was then identified by specific antibodies in an enzyme immunoassay (EIA). The LLC-MK2 and MDCK cell lines were found to provide optimal growth of parainfluenza and influenza viruses respectively. During the period from November, 1990 to July, 1992, 6501 respiratory specimens were tested. There were 100 influenza A, 36 influenza B and 261 parainfluenza virus isolates. The influenza isolates were further subtyped by the WHO Influenza Reference Centre. The use of these cell lines and the EIA provided an effective method for the routine culture of these viruses.

References

Sep 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·F L RubenR E Sabbagha
Jan 1, 1988·Annals of the New York Academy of Sciences·R B Kundsin, S A Poulin
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R W Compans, P W Choppin
Feb 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·F B GORDON, A L QUAN

Citations

Dec 1, 1994·Journal of Virological Methods·T W KokC J Burrell
Mar 26, 2004·Journal of Virological Methods·Belinda StoneT W Kok
Oct 24, 1998·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·T W KokL Payne
Feb 19, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·T W KokG Higgins
Oct 20, 1998·Australian and New Zealand Journal of Medicine·S K SchepetiukM Qiao
Oct 2, 2013·Antimicrobial Agents and Chemotherapy·Daisuke TamuraL V Gubareva
Jan 1, 1995·Scandinavian Journal of Infectious Diseases·S K SchepetiukT W Kok
Apr 16, 2015·Human Vaccines & Immunotherapeutics·Nagendra R Hegde
Sep 23, 2014·Diagnostic Microbiology and Infectious Disease·Laurent BussonOlivier Vandenberg
Jul 8, 2018·Molecular & Cellular Proteomics : MCP·Andrea C BeckerJörn Dengjel
Dec 27, 2019·Mass Spectrometry Reviews·Aleksandra MilewskaPiotr Suder
Apr 20, 2018·Human Vaccines & Immunotherapeutics·Alberto Pérez Rubio, Jose María Eiros
Oct 27, 2017·Scientific Reports·Andrey V MarkovEvgeniya B Logashenko

Related Concepts

Metazoa
Peroxidase-Antiperoxidase Complex Technique
Influenza
Myxoviruses
Respiratory Tract Structure
Virus Cultivation
Virus Replication
Morphologic Artifacts
Respirovirus
Parainfluenza

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