The use of microporous divinyl benzene copolymer for yeast cell immobilization and ethanol production in packed-bed reactor

Applied Biochemistry and Biotechnology
Pinar KaragözMelek Ozkan

Abstract

Microporous divinyl benzene copolymer (MDBP) was used for the first time as immobilization material for Saccharomyces cerevisiae ATCC 26602 cells in a bed reactor and ethanol production from glucose was studied as a model system. A very homogenous thick layer of yeast cells were seen from the scanning electron micrographs on the outer walls of biopolymer. The dried weight of the cells was found to be approximately 2 g per gram of cell supporting material. Hydrophobic nature of polymer is an important factor increasing cell adhesion on polymer pieces. The dynamic flow conditions through the biomaterial due to its microporous architecture prevented exopolysaccharide matrix formation around cells and continuous washing out of toxic metabolites and dead and degraded cells from the reactor provided less diffusional limitations and dynamic living environment to the cells. In order to see the ethanol production performance of immobilized yeast cells, a large initial concentration range of glucose between 6.7 and 300 g/l was studied at 1 ml/min in continuous packed-bed reactor. The inhibition effect of glucose with increasing initial concentration was observed at above 150 g/l, a relatively high substrate concentration. The continuous ...Continue Reading

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Citations

Jul 16, 2010·Biotechnology Advances·Solange I MussattoJosé A Teixeira
Jun 1, 2010·Applied Biochemistry and Biotechnology·Eleonora WinkelhausenSlobodanka Kuzmanova
Mar 16, 2011·Biotechnology and Bioengineering·Daniel SchäpperKrist V Gernaey
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Sheida StephensD Grant Allen

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