The use of mini-organ cultures of human upper aerodigestive tract epithelia in ecogenotoxicology

Mutation Research
Norbert H KleinsasserAlbert W Rettenmeier

Abstract

The carcinogenic potential of xenobiotics and possible confounders are often difficult to differentiate in in vivo studies. In contrast, in vitro studies allow investigation of the impact of carcinogens on human target cells under standardized conditions. The aim of the present study is to demonstrate whether three-dimensional mini organ-cultures (MOCs) of human inferior nasal turbinate epithelia may represent a useful model to study genotoxic effects of xenobiotics in vitro. Culture of mini organs was performed by cutting 1mm3 pieces from fresh specimens of inferior nasal turbinates. After a period of 5-6 days the specimens were fully covered with epithelium. On days 7, 9, and 11 of culture, intact MOCs from 25 tissue donors were incubated with dimethyl sulfoxide (DMSO) as a negative control, or with mono(2-ethylhexyl) phthalate (MEHP), benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). On days 7 and 11, MOCs were analyzed by the alkaline Comet assay to detect DNA-single-strand breaks, alkali-labile sites and incomplete excision-repair sites. DNA migration after single exposure of non-cultivated fresh specimens was also analyzed. In order to detect regimen-specific effects, DNA fragmen...Continue Reading

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Citations

Sep 20, 2005·HNO·N H KleinsasserE Richter
May 5, 2007·Archives of Toxicology·Katharina SchmidNorbert Kleinsasser
Apr 23, 2008·Cell Biology and Toxicology·Alok DhawanDevendra Parmar
Jun 13, 2012·Oxidative Medicine and Cellular Longevity·Philipp BaumeisterUlrich Harréus
Jul 24, 2008·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Norbert H KleinsasserMichael Buehrlen
Dec 4, 2012·Mutation Research·Christian GinzkeyNorbert H Kleinsasser
May 15, 2010·Toxicology Letters·Christian GinzkeyNorbert H Kleinsasser
Dec 17, 2008·Cancer Detection and Prevention·P BaumeisterU Harréus
Jul 26, 2011·Environmental and Molecular Mutagenesis·Stephan HackenbergNorbert Kleinsasser
May 25, 2013·Inhalation Toxicology·Christian KoehlerNorbert Helmut Kleinsasser
Jan 1, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S SwitallaK Sewald
Dec 23, 2008·Biochemical and Biophysical Research Communications·Seong Kook ParkYoung-Il Yang
Dec 17, 2014·Frontiers in Genetics·Emilio RojasMahara Valverde
Apr 29, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Norbert H KleinsasserElmar Richter
Aug 27, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Andrea W SassenNorbert H Kleinsasser
Jun 6, 2009·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Philipp BaumeisterUlrich Harréus
Jun 12, 2014·Clinical and Experimental Otorhinolaryngology·Tae-Hoon LeeSeong Kook Park

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