The use of near infrared analysis for in situ studies

Journal of Dairy Science
P BerzaghiI Andrighetto

Abstract

This study evaluated the use of near infrared reflectance spectroscopy to estimate the chemical composition of feed residues after in situ ruminal incubation. Residues of three alfalfa hays (n = 93) and three alfalfa pellets (n = 93) obtained after ruminal exposure were analyzed for dry matter, crude protein, neutral detergent fiber, acid detergent fiber, and acid detergent lignin by wet chemistry and were also scanned with a near infrared monochromator instrument. A calibration was calculated that combined hay and pellet samples (n = 60). Validation tests were performed using the remaining feed residues. The coefficients of determination and standard errors (percentage of dry matter) of the validation tests for crude protein, neutral detergent fiber, acid detergent fiber, and lignin were of 0.95 and 0.92, 0.96 and 1.68, 0.95 and 1.56, and 0.99 and 0.48, respectively. Similar statistics were obtained using the SELECT algorithm of sample selection; a further 30% reduction was observed in the number of samples that were used for calibration. Kinetics of ruminal degradation and effective degradabilities that were calculated based on chemical composition of the residues as determined by wet chemistry or estimated by near infrared r...Continue Reading

References

Dec 1, 1979·The Proceedings of the Nutrition Society·I M Morrison
Aug 1, 1991·Journal of Dairy Science·J B ReevesJ Vandersall
Sep 1, 1980·Journal of Dairy Science·D R Mertens, J R Loften
Sep 1, 1993·Journal of Dairy Science·I AndrighettoD Sapienza

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