PMID: 2508747Nov 6, 1989Paper

The use of newly synthesized phospholipids for assembly into secreted hepatic lipoproteins

Biochimica Et Biophysica Acta
J E Vance

Abstract

We have recently shown (Vance, J.E. (1988) Biochim. Biophys. Acta 963, 70-81) that the percent distribution of molecular species of phosphatidylcholine (PC) derived from [methyl-3H]choline and [3-3H]serine, and phosphatidylethanolamine (PE) derived from [3-3H]serine were different in secreted lipoproteins and in the cultured hepatocytes from which the lipoproteins were produced. The species 1-stearoyl-2-arachidonoyl PC and PE were selectively not secreted. How this selection occurs is not known. One possible explanation is that secreted phospholipids are representative of the newly synthesized pool, whereas the molecular species composition of bulk cellular phospholipids has been altered by selective deacylation or by deacylation-reacylation. This hypothesis has been tested. The percent distribution of radioactivity from [1-3H]ethanolamine, [3-3H]serine and [methyl-3H]choline in nascent cellular and secreted PE and PC molecular species was examined by high-performance liquid chromatography. From [3H]serine labeling, the percent distribution of [3H]PE species in the medium after 4 h resembled closely that in cells 0.5 h, but not 4 h, after labeling. Thus, nascent phosphatidylserine-derived PE was immediately earmarked for secret...Continue Reading

References

May 1, 1976·The Journal of Cell Biology·C A AlexanderR J Havel
Jun 9, 1970·Biochimica Et Biophysica Acta·B AkessonG Arvidson
May 1, 1968·European Journal of Biochemistry·G A Arvidson
Jan 17, 1984·Biochimica Et Biophysica Acta·D E VanceD Steinberg
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D R Voelker
Jan 1, 1965·Annual Review of Biochemistry·W E LANDS

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Citations

Jun 15, 1990·Experientia·J E Vance, D E Vance
Jul 1, 1990·The Journal of Nutritional Biochemistry·S H Zeisel
Jun 18, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G C Burdge, A D Postle
Jun 18, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M VázquezJ C Laguna
Apr 13, 1999·Gastroenterology Clinics of North America·A D Cooper
Feb 1, 2012·International Journal of Cell Biology·Leanne PereiraMarica Bakovic
Aug 1, 1993·Current Opinion in Cell Biology·G van Meer
May 5, 1999·Chemico-biological Interactions·N KudoY Kawashima
Feb 26, 2015·Autophagy·Joanna BiazikEeva-Liisa Eskelinen
Oct 4, 2011·Medical Hypotheses·J P GirardiM Bakovic
Dec 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·S L Abidi

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