PMID: 11899108Mar 20, 2002Paper

The use of on-line and off-line chromatographic extraction techniques coupled with mass spectrometry for support of in vivo and in vitro assays in drug discovery

Current Topics in Medicinal Chemistry
J J A MasucciM Evangelisto

Abstract

We have investigated various sample chromatographic extraction and sample preparation methods for liquid chromatography mass spectrometry analysis in order to increase the throughput of various in vivo and in vitro assays in support of drug discovery. The results indicated that direct plasma injection, although certainly faster than conventional protein precipitation for sample preparation, had problems associated with column longevity and overall robustness. Frequently a single study could not be completed without column replacement. On-line solid phase extraction, on the other hand, compared well with off-line solid phase extraction, using our LC extraction column design, as contamination of the extraction column was minimized by back flushing using the Gilson syringe pump. Finally, on-line solid phase extraction for support of Caco-2 permeability studies worked very well for both single components and mixtures as the matrix was much simpler, presenting fewer contamination problems.

Citations

Jul 14, 2005·European Journal of Drug Metabolism and Pharmacokinetics·Gary W CaldwellWilliam Jones
Jul 6, 2010·Clinical Biochemistry·Michael Vogeser, Fabian Kirchhoff
Apr 15, 2008·Drug Discovery Today·Gerhard F EckerPeter Chiba
Jul 22, 2008·Journal of Veterinary Pharmacology and Therapeutics·M MartinezK Mealey
Feb 3, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jean-Louis DelaunayMichèle Maurice
Jun 11, 2020·International Journal of Molecular Sciences·Liadys Mora LagaresMarjana Novič
Mar 8, 2012·British Journal of Pharmacology·A S GandhiR Ghose
Apr 4, 2007·Mass Spectrometry Reviews·Chandra PrakashAngus Nedderman
Dec 17, 2014·Pharmacology Research & Perspectives·Olga AnderssonGunnar Andersson
May 28, 2016·Journal of Alzheimer's Disease : JAD·Alexandre V IvachtchenkoIlya Okun

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