The use of phage FCL-2 as an alternative to chemotherapy against columnaris disease in aquaculture

Frontiers in Microbiology
Elina LaantoLotta-Riina Sundberg

Abstract

Flavobacterium columnare, the causative agent of columnaris disease in fish, causes millions of dollars of losses in the US channel catfish industry alone, not to mention aquaculture industry worldwide. Novel methods are needed for the control and treatment of bacterial diseases in aquaculture to replace traditionally used chemotherapies. A potential solution could be the use of phages, i.e., bacterial viruses, host-specific and self-enriching particles that can be can easily distributed via water flow. We examined the efficacy of phages to combat columnaris disease. A previously isolated phage, FCL-2, infecting F. columnare, was characterized by sequencing. The 47 142 bp genome of the phage had G + C content of 30.2%, and the closest similarities regarding the structural proteins were found in Cellulophaga phage phiSM. Under controlled experimental conditions, two host fish species, rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio), were used to study the success of phage therapy to prevent F. columnare infections. The survival of both fish species was significantly higher in the presence of the phage. Hundred percent of the zebrafish and 50% of the rainbow trout survived in the phage treatment (survival without ...Continue Reading

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Citations

Mar 16, 2017·BMC Evolutionary Biology·Hanna KinnulaLotta-Riina Sundberg
Mar 3, 2018·Pharmaceuticals·Teagan L BrownJoseph Tucci
Jan 27, 2018·Archives of Microbiology·Constantina KokkariPantelis Katharios
Mar 25, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ville HoikkalaLotta-Riina Sundberg
Jan 12, 2019·Annual Review of Food Science and Technology·Lisa O'SullivanAidan Coffey
Dec 7, 2019·Microbiology Resource Announcements·Janne J RavanttiLotta-Riina Sundberg
Jun 4, 2020·Antibiotics·Elina LaantoLotta-Riina Sundberg
May 19, 2017·Evolutionary Applications·Hanna KinnulaLotta-Riina Sundberg
Jun 10, 2017·Genome Announcements·Elina LaantoLotta-Riina Sundberg
Jul 26, 2017·Nature Communications·Elina LaantoLotta-Riina Sundberg
Jan 8, 2021·PHAGE : Therapy, Applications, and Research·Krister SundellTom Wiklund
Aug 26, 2017·Microbiology Spectrum·Stephen T Abedon

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Datasets Mentioned

BETA
KM873719

Methods Mentioned

BETA
transmission electron microscopy
454 sequencing

Software Mentioned

BLAST
Ion Torrent
Glimmer
GS De Novo Assembler
GeneMarkS
SPSS
Geneious

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