The use of photoactivatable reagents for the study of cell lineage in Drosophila embryogenesis

Methods in Cell Biology
C H Girdham, P H O'Farrell

Abstract

Photoactivatable lineage tracers represent a major advance for clonal analysis in the early embryo and the study of cell movements. Any cell in the blastoderm can be marked, and the nuclear localization of the signal allows excellent resolution in identifying the daughters of individual cells. Although the technique is limited by the availability of the water-soluble caged fluorescein and its derivatives for synthesis of the complete tracer, these may become commercially available in the future. The use of caged rhodamine derivatives or antibody amplification of the signal may greatly extend the developmental period over which marked clones can be identified.

Citations

Aug 31, 2016·Chemistry : a European Journal·Yang ZhangFrançisco M Raymo
Apr 5, 2007·Nature Protocols·Kohei HattaTomomi Omura
Jun 1, 1996·Current Opinion in Neurobiology·J M Nerbonne
Jun 14, 2002·Current Biology : CB·Sven PfeifferJean-Paul Vincent

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