PMID: 18705621Aug 19, 2008Paper

The use of polymerase chain reaction assay versus conventional methods in detecting neonatal chlamydial conjunctivitis

Journal of Pediatric Ophthalmology and Strabismus
Pui-pui YipChi-kin Ho

Abstract

To compare the performance of polymerase chain reaction versus conventional methods (cell culture and direct immunofluorescent assay) in diagnosing neonatal chlamydial conjunctivitis and their correlations to the severity of conjunctivitis. Consecutive cases of neonatal conjunctivitis were recruited over a year. Both eyes were clinically graded according to the severity of conjunctivitis and investigated using the three aforementioned chlamydial tests. Neonatal chlamydial conjunctivitis was assumed if one of these three tests was positive and there was clinical improvement after treatment. Sensitivity and specificity of each of the tests were analyzed. Three hundred sixty-eight sets of chlamydial tests were done for 184 neonates. The percentage of positive results was 93.8% and 71.9% for polymerase chain reaction and conventional methods, respectively. Using positive results in either cell culture or direct immunofluorescent assay as a standard to diagnose neonatal chlamydial conjunctivitis, the sensitivity and specificity of polymerase chain reaction were 92.0% and 97.7%, respectively. If we used polymerase chain reaction as a standard, the sensitivity and specificity of cell culture were 73.3% and 99.7%, respectively. A discr...Continue Reading

References

Dec 15, 1992·American Journal of Ophthalmology·A R TalleyJ S Pepose
May 1, 1990·Journal of Clinical Microbiology·C A CrouseS S Atherton
Aug 1, 1996·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·E M HallerK Muellner
Aug 25, 1999·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·V BersudskyS Rumelt

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Citations

Mar 3, 2011·Current Infectious Disease Reports·Onsiri Thanathanee, Terrence P O'Brien
Dec 7, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Margaret R Hammerschlag
Apr 14, 2012·Journal français d'ophtalmologie·K D AyenaK P Balo
Sep 23, 2020·The Cochrane Database of Systematic Reviews·Vimal Scott KapoorS Swaroop Vedula

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