PMID: 7014913May 1, 1981Paper

The use of polystyrene microcarriers to prepare cell monolayers for transmission electron microscopy

Journal of Microscopy
G F SargentA S McNeish

Abstract

A simple method is described, using polystyrene microcarrier beads, that allows cell monolayers to be prepared without distortion for transmission electron microscopy. Cells cultured on polystyrene beads (Biosilon R, Nunc, Kamstrup, Denmark) are held in an agar matrix. Before embedding, the beads are dissolved in 1,2-epoxypropane. The technique increased the number of observable cells, when compared to preparations of planar monolayers, and there is good preservation of morphological detail.

References

May 1, 1977·Stain Technology·W NopanitayaJ W Grisham
Oct 1, 1967·The Journal of Cell Biology·B R BrinkleyL C Richardson
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Apr 1, 1972·Journal of Ultrastructure Research·J Lipetz, V J Cristofalo

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Citations

Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A B MurrayE Blauw

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