PMID: 20650132Oct 1, 1995Paper

The use of pre-implantation mouse embryos cultured in vitro in toxicological studies

Toxicology in Vitro : an International Journal Published in Association with BIBRA
L A ClericiA Collotta

Abstract

The preimplantation mouse embryo has been found to be a good model for various toxicological investigations. This communication deals with two examples of research activities carried out in our laboratory to detect the embryotoxic properties of tritium and of 1,2:3,4-diepoxybutene (DEB). Exposures of blastocysts for 24 hr to concentrations as high as 0.296 kBq/ml tritiated amino acids or nucleoside induced a statistically significant reduction in the percentage of embryos that reached the stage of two-layer inner cell mass (ICM). The same quantity of tritiated arginine, but not of thymidine or tryptophan, also induced a lower percentage of differentiating ICM than the control when added to culture medium during the second cleavage division. These findings support the idea that tritium released by nuclear power plants as HT or as tritiated water (HTO) could become a radiotoxicological problem since it can be converted easily into organic compounds by living organisms. DEB was found to be highly embryotoxic in preimplantation mouse embryos in vitro at micromolar concentrations. This compound is formed in mammalian cells by the oxidative metabolism of 1,3-butadiene, a chemical used in rubber industries and present in tobacco smoke...Continue Reading

References

Oct 15, 1975·Experimental Cell Research·R A Pedersen, J E Cleaver
Jun 1, 1990·Environmental Health Perspectives·R E MorrisseyT J Mast
Aug 1, 1985·The Journal of Experimental Zoology·A SpindleR A Pedersen
Aug 10, 1973·Nature·C R Austin
Dec 1, 1982·Experimental Cell Research·A SpindleR A Pedersen
Aug 1, 1980·In Vitro·A Spindle

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