PMID: 13575674Sep 1, 1958Paper

The use of precipitin analysis in agar for the study of human streptococcal infections. III. The purification of some of the antigens detected by these methods

The Journal of Experimental Medicine
S P HALBERT

Abstract

As evidenced by precipitin analysis with pooled human gamma globulin, at least 12 distinct antigens were produced in cultures by one strain of Group A streptococcus (C203S). It was suggested on this basis, that these antigens were produced in vivo during human infections. By the combined use of continuous flow electrophoresis on paper curtains, and column chromatography with calcium phosphate gels, five of these have been isolated in a probable high state of purity. One of the components was obtained from culture filtrates of a Group C streptococcal strain. Three of the purified antigens have been tentatively identified as streptolysin "O", diphosphopyridinenucleotidase, and proteinase precursor. The latter could be very readily crystallized, and appears "identical" with that described by Elliott. The DPNase was of extremely high potency, 1 mg. being capable of destroying 12.6 gm. of DPN in 7(1/2) minutes at 37 degrees C. The identity of the other two components is uncertain as yet. They are distinct from each other and the above products immunologically, and are not related to the "C" carbohydrate. The applicability of these methods for the analysis of infectious diseases generally was discussed.

References

Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·T N HARRISC A OGBURN
Nov 1, 1956·Archives of Biochemistry and Biophysics·S HJERTENA TISELIUS
Dec 1, 1957·A.M.A. Archives of Ophthalmology·C TANAKA
Dec 16, 1957·Annals of the New York Academy of Sciences·P GRABAR
Jun 1, 1958·The Journal of Experimental Medicine·L W WANNAMAKER
Dec 1, 1949·The Journal of Experimental Medicine·M McCARTY

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Citations

Jan 21, 1961·Nature·S P HALBERTE DAHLE
Apr 1, 1961·The Journal of Experimental Medicine·S P HALBERTE DAHLE
Mar 1, 1965·The Journal of Experimental Medicine·H C DILLON, L W WANNAMAKER
Dec 1, 1965·The Journal of Experimental Medicine·W B DandlikerH C Schapiro
Apr 1, 1970·The Journal of Experimental Medicine·A ThompsonU Smith
Jan 1, 1980·Pharmacology & Therapeutics·J E Alouf
Jan 1, 1966·Acta Pathologica Et Microbiologica Scandinavica·B NygrenP Wåhlen
Jan 1, 1961·Acta Pathologica Et Microbiologica Scandinavica·L A HANSON, S E HOLM
Jan 1, 1971·European Journal of Biochemistry·F J Fehrenbach
Jun 1, 1959·Journal of Bacteriology·W A PIERCE
Mar 1, 1973·Infection and Immunity·D E Van Epps, B R Andersen

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