The use of real-time quantitative PCR to detect circulating prostate-specific membrane antigen mRNA in patients with prostate carcinoma

Annals of the New York Academy of Sciences
Da-Chang ChuChien-Feng Sun

Abstract

Prostate-specific antigen (PSA) has long been criticized for its lack of specificity in screening for the occurrence of prostate cancer. In this study, we tried to measure levels of another biomarker, prostate-specific membrane antigen (PSM), in the peripheral circulation from subjects with either prostate cancer or benign prostatic hyperplasia (BPH). Total RNA was extracted from blood samples of 70 patients with prostate cancer and 19 with BPH. Reverse transcription was performed to convert mRNA to cDNA. The cDNA was analyzed with a novel real-time quantitative polymerase chain reaction (PCR) protocol to measure PSM mRNA levels in the circulation. Melting curve analysis was adapted to assure that correct amplification data were obtained. Results showed that 41 of 70 prostate cancer patients had positive results, whereas 9 of 19 BPH cases were negative. Therefore, the sensitivity and specificity were determined to be 58.6% and 47.4%, respectively. For comparison, traditional nested PCR was performed to investigate whether the new method was superior. The sensitivity and specificity of nested PCR were determined to be 27.1% and 57.9%, respectively. The detection limits of these two methods were 0.0005 ng (for the real-time quant...Continue Reading

Citations

Sep 30, 2005·Virchows Archiv : an International Journal of Pathology·Giuseppe PelosiGiuseppe Viale
Jan 30, 2008·Prostate Cancer and Prostatic Diseases·M L RamírezC P Evans
May 31, 2014·BioMed Research International·Andreas DimakakosMichael Koutsilieris
Dec 2, 2006·Urologic Oncology·Timothy J BradfordArul M Chinnaiyan
Apr 25, 2007·Pathology·Jason C H Tsang, Y M Dennis Lo
Feb 27, 2010·Cellular & Molecular Biology Letters·Marketa UrbanovaJan Betka
Nov 28, 2018·Nature Reviews. Urology·Emma E van der ToomKenneth C Valkenburg
Oct 20, 2006·Critical Reviews in Clinical Laboratory Sciences·Qinghua FengNancy B Kiviat
Aug 1, 2015·World Journal of Gastroenterology : WJG·Takahiro KishikawaKazuhiko Koike

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