The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations.

Virology Journal
Andrea C Baker, Declan C Schroeder

Abstract

Single-stranded RNA viruses, infectious to the European honeybee, Apis mellifera L. are known to reside at low levels in colonies, with typically no apparent signs of infection observed in the honeybees. Reverse transcription-PCR (RT-PCR) of regions of the RNA-dependent RNA polymerase (RdRp) is often used to diagnose their presence in apiaries and also to classify the type of virus detected. Analysis of RdRp conserved domains was undertaken on members of the newly defined order, the Picornavirales; focusing in particular on the amino acid residues and motifs known to be conserved. Consensus sequences were compiled using partial and complete honeybee virus sequences published to date. Certain members within the iflaviruses, deformed wing virus (DWV), Kakugo virus (KV) and Varroa destructor virus (VDV); and the dicistroviruses, acute bee paralysis virus (ABPV), Israeli paralysis virus (IAPV) and Kashmir bee virus (KBV), shared greater than 98% and 92% homology across the RdRp conserved domains, respectively. RdRp was validated as a suitable taxonomic marker for the assignment of members of the order Picornavirales, with the potential for use independent of other genetic or phenotypic markers. Despite the current use of the RdRp a...Continue Reading

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Citations

Sep 29, 2009·Applied and Environmental Microbiology·Andrea C HighfieldDeclan C Schroeder
Oct 16, 2010·PloS One·Jerry J BromenshenkRobert A Cramer
Jun 17, 2008·Current Opinion in Microbiology·Michael J Allen, William H Wilson
Jul 13, 2013·Virus Research·Abby L LevittEdward C Holmes
Mar 29, 2011·Journal of Invertebrate Pathology·Clare AllenCharles A Strong
May 19, 2017·Journal of Virology·Emily J RemnantAlyson Ashe
Oct 28, 2015·The ISME Journal·Gideon J MordecaiDeclan C Schroeder

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Datasets Mentioned

BETA
AAA47928
Foot
CAB40546
AAK12387
AAZ73355
AAK13619
AAU10094
AAL79021

Methods Mentioned

BETA
PCR

Software Mentioned

ClustalW
BLAST

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