The use of routine special stains for upper gastrointestinal biopsies

The American Journal of Surgical Pathology
Cheryl L Wright, James K Kelly

Abstract

Helicobacter pylori and intestinal metaplasia (IM) are readily seen in hematoxylin and eosin-stained slides of gastric and/or esophageal biopsies, yet many pathology laboratories perform routine special stains on all of these biopsies. We wished to determine if special stains are necessary for every single gastric and/or esophageal biopsy. We prospectively studied 613 gastric and/or esophageal biopsies from 494 consecutive patients. The slides were stained with hematoxylin and eosin, toluidine blue (TB) for H. pylori, and Alcian blue (AB) for IM. The hematoxylin and eosin slide was classed as positive or negative for H. pylori and IM. Then it was determined if the case needed a TB or AB stain. A total of 436 cases (71.1%) were identified as H. pylori-negative and not needing a TB stain, and none was TB+. A total of 126 (20.6%) of hematoxylin and eosin slides were inconclusive for H. pylori and were regarded as needing a TB stain. Twenty of these (15.9%) were TB+. Fifty-one biopsies (8.3%) were regarded as H. pylori+ on hematoxylin and eosin; the TB stain was also positive in 49. IM was present in 113 (18.4%) hematoxylin and eosin biopsies. Hematoxylin and eosin slides were IM-negative in 498 cases (81.2%). The AB stain revealed...Continue Reading

Citations

Jul 22, 2016·Scandinavian Journal of Gastroenterology·Edith LahnerBruno Annibale
Apr 24, 2012·American Journal of Clinical Pathology·S Brooks SmithShadi A Qasem
Aug 22, 2009·Histopathology·Michael KoenigNeil A Shepherd
Aug 24, 2006·Helicobacter·Katarzyna Dzierzanowska-FangratDanuta Dzierzanowska
Aug 5, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Yu YangLanjing Zhang
Dec 17, 2014·American Journal of Clinical Pathology·Yoginder Chitkara
Oct 22, 2013·The American Journal of Surgical Pathology·Kenneth P BattsUNKNOWN Rodger C Haggitt Gastrointestinal Pathology Society
Dec 1, 2018·Applied Immunohistochemistry & Molecular Morphology : AIMM·Jung H SonStephen M Lagana
Jul 10, 2019·Southern Medical Journal·Benjamin R Lloyd, David A Leiman
Apr 21, 2016·The American Journal of Surgical Pathology·Nicole C Panarelli, Rhonda K Yantiss
Jul 13, 2018·Digestive Diseases and Sciences·Robert Odze
Jan 4, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Magnus HallandWilliam A Petri
Feb 11, 2021·Romanian Journal of Internal Medicine = Revue Roumaine De Médecine Interne·Alfred NelsonDana Harris
Nov 30, 2020·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Kenneth W Hung, Daniel E Freedberg
May 6, 2021·Journal of Primary Care & Community Health·Pedro CortésDana M Harris
Jul 6, 2021·Frontiers in Oncology·Lu ZhangMuhan Lü

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