The use of serum spotted onto filter paper for diagnosing and monitoring Chikungunya virus infection

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Séverine MatheusIsabelle Leparc-Goffart

Abstract

The recent emergence of Chikungunya Virus (CHIKV) in the Americas constitutes a major public health problem on this continent, where the mosquito vector is widespread. The rapid diagnosis of suspected cases is essential for the monitoring and control of this ongoing outbreak. However, this requires reliable tools that are difficult to establish in areas without specialized laboratories. The aim was to evaluate the performances of serum samples spotted onto filter paper for molecular and serological diagnosis of Chikungunya infection. Analyses were performed from frozen sera and serum spotted onto filter paper provided from 121 Chikungunya suspected cases collected at a biological laboratory on Saint-Martin Island. This approach performed well in comparisons with standard methods, with a sensitivity of 100% and a specificity of 93.6% for the combined technical approaches (RT-PCR and serological results). Comparisons of serum samples spotted onto filter paper and frozen samples showed a concordance rate of 94.8% in molecular tests and 98.2% in serological tests. This simple sampling technique could overcome the problems of the lack of efficient CHIKV diagnosis tools in remote regions, providing good results regardless of the mole...Continue Reading

References

Mar 2, 2005·Journal of Virological Methods·Irina PradoMaría G Guzmán
May 14, 2005·Emerging Infectious Diseases·Christophe N PeyrefitteHugues J Tolou
Jan 19, 2007·Journal of Clinical Microbiology·Séverine MatheusXavier Deparis
Apr 24, 2007·The Lancet Infectious Diseases·Gilles PialouxMichel Strobel
Oct 9, 2007·Pathologie-biologie·P GrivardA Michault
Mar 8, 2008·Emerging Infectious Diseases·Marcus PanningChristian Drosten
Apr 6, 2011·Current Infectious Disease Reports·Fabrice SimonCatherine Marimoutou
Jun 15, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Maite AubryVan-Mai Cao-Lormeau
Aug 13, 2013·PLoS Neglected Tropical Diseases·Soa Fy AndriamandimbyVincent Richard
Jan 29, 2014·The American Journal of Tropical Medicine and Hygiene·Séverine MatheusVincent Marechal
Feb 11, 2014·Lancet·Isabelle Leparc-GoffartXavier de Lamballerie
Apr 12, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·S CassadouM Ledrans
Apr 12, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·R OmarjeeI Leparc-Goffart
May 16, 2015·BMC Medicine·Marcio Roberto Teixeira NunesPedro Fernando da Costa Vasconcelos

❮ Previous
Next ❯

Citations

Apr 19, 2019·Journal of Clinical Microbiology·Muktha S NatrajanJesse J Waggoner
Apr 14, 2018·Vector Borne and Zoonotic Diseases·So Lee ParkDana L Vanlandingham

❮ Previous
Next ❯

Related Concepts