The use of silk fibroin/hydroxyapatite composite co-cultured with rabbit bone-marrow stromal cells in the healing of a segmental bone defect

The Journal of Bone and Joint Surgery. British Volume
G WangX Cai

Abstract

In a rabbit model we investigated the efficacy of a silk fibroin/hydroxyapatite (SF/HA) composite on the repair of a segmental bone defect. Four types of porous SF/HA composites (SF/HA-1, SF/HA-2, SF/HA-3, SF/HA-4) with different material ratios, pore sizes, porosity and additives were implanted subcutaneously into Sprague-Dawley rats to observe biodegradation. SF/HA-3, which had characteristics more suitable for a bone substitute based on strength and resorption was selected as a scaffold and co-cultured with rabbit bone-marrow stromal cells (BMSCs). A segmental bone defect was created in the rabbit radius. The animals were randomised into group 1 (SF/HA-3 combined with BMSCs implanted into the bone defect), group 2 (SF/HA implanted alone) and group 3 (nothing implanted). They were killed at four, eight and 12 weeks for visual, radiological and histological study. The bone defects had complete union for group 1 and partial union in group 2, 12 weeks after operation. There was no formation of new bone in group 3. We conclude that SF/HA-3 combined with BMSCs supports bone healing and offers potential as a bone-graft substitute.

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Citations

Nov 10, 2012·Advanced Drug Delivery Reviews·Banani KunduXungai Wang
May 16, 2013·Journal of Materials Science. Materials in Medicine·Ning ChengBin Shi
Jul 30, 2011·Journal of Tissue Engineering and Regenerative Medicine·Eugene Yong-Shun SeeJames Cho Hong Goh
Feb 15, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Fuzhan ZhangHuilin Yang
Nov 28, 2012·Advanced Healthcare Materials·Naresh Kasoju, Utpal Bora
Jun 4, 2020·Science and Technology of Advanced Materials·Muhammad SaleemChen Yougen
Sep 11, 2012·International Journal of Molecular Medicine·Jeon-Woo ChonYoo Kyoung Park
Nov 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiao Jiao LiHala Zreiqat
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Feb 18, 2021·ACS Biomaterials Science & Engineering·Li ZhangWeiliang Shen
Mar 23, 2021·Critical Reviews in Biotechnology·Hai-Yan WangZheng-Guo Wei
Jul 18, 2020·ACS Nano·Kenneth S Ogueri, Cato T Laurencin

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