The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapanemase-producing Escherichia coli under antibiotic pressure

Scientific Reports
Hanna E SidjabatGerhard Schenk

Abstract

Antibiotic resistance associated with the clinically significant carbapenemases KPC, NDM and OXA-48 in Enterobacteriaceae is emerging as worldwide. In Australia, IMP-producing Enterobacteriaceae are the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such CPE are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli strains producing either NDM-, IMP- or KPC-type carbapenemases were included in this study, and their proteomes were analysed in growth conditions with or without meropenem. The most significant difference in the bacterial proteomes upon the addition of meropenem was triggered amongst NDM-producers and to a lower extent amongst KPC-producers. In particular, HU DNA-binding proteins, the GroEL/GroES chaperonin complex and GrpE proteins were overexpressed. These proteins may thus contribute to the better adaptability of NDM- and KPC-producers to meropenem. A significa...Continue Reading

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Citations

Jul 28, 2018·FEMS Microbiology Letters·Vladislav M ChernovMaxim V Trushin
Sep 2, 2020·Pharmaceuticals·Foteini TsakouBiljana Mojsoska
Dec 3, 2020·International Journal of Antimicrobial Agents·Olga A ChernovaRustam I Aminov

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Datasets Mentioned

BETA
PXD008019

Methods Mentioned

BETA
PCA
protein folding
PCR

Software Mentioned

SWATH
Markerview
PeakView
ProteinPilot
Paragon
Mascot

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