PMID: 6974751Jan 1, 1981Paper

The use of tannic acid fixation for the electron microscope visualization of fluorochrome-labelled antibodies attached to cell surface antigens

Journal of Immunological Methods
P J RohollJ G Vos

Abstract

Tannic acid as a prefixative for EM purposes was introduced by Futaesaku et al. (1972). The fixative creates conditions for enhancing electron density of different protein materials. By using a mixture of tannic acid and glutaraldehyde as prefixative, followed by a routine procedure of postfixation (OsO4) and poststaining (uranylacetate and lead citrate), membrane bound antibodies not conjugated with electron dense markers are made visible under the electron microscope.

References

Nov 1, 1976·Journal of Ultrastructure Research·R C Wagner
Sep 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D M BroorsmaN Kors
Dec 1, 1979·European Journal of Immunology·B J BastR E Ballieux
Feb 1, 1975·Journal of Ultrastructure Research·B van Deurs
Mar 1, 1974·European Journal of Immunology·F Loor
Mar 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger

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Citations

Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hisham Bazzi, Kathryn V Anderson
Apr 25, 2007·Issues in Mental Health Nursing·Linda Stafford
Nov 1, 1990·Ultrastructural Pathology·K P Dingemans, M A van den Bergh Weerman

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