The use of the hydrodynamic HBV animal model to study HBV biology and anti-viral therapy

Hepatology Research : the Official Journal of the Japan Society of Hepatology
Mali Ketzinel-GiladE Galun

Abstract

A simple reproducible and versatile small animal model for hepatitis B virus (HBV) infection is still unavailable. We have generated a simple transient liver-targeted transgenic mouse. Hydrodynamics tail vein injection of a head-to-tail dimer of adw HBV genome (pHBVadwHTD) into immunocompetent mice generated HBsAg and HBeAg expression in both serum and hepatocytes, followed by seroconversion. The injection of pHBVadwHTD into SCID mice generated prolonged HBsAg and HBeAg antigenemia and HBV viremia. Our results demonstrate that hydrodynamic injection of naked DNA could support the generation of HBV particles. We used this model for the assessment of anti-viral agents. Administration of our human monoclonal antibodies, HBV-Ab17(XTL) and HBV-Ab19(XTL), as well as Lamivudine (3TC) treatment suppressed HBV viremia. The model presented herein supports long and stable expression of HBV and will enable determination of various biological questions related to HBV life cycle, mutants and could enhance the development of anti-viral reagents.

Citations

Oct 4, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Takeshi Suda, Dexi Liu
Jun 26, 2007·Acta Pharmacologica Sinica·Jin-liang PengYu-hong Xu
Mar 10, 2015·International Journal of Molecular Sciences·Xiaosong LiAilong Huang
Jan 17, 2007·Expert Opinion on Biological Therapy·Gloria Gonzalez-AseguinolazaAstrid Paneda
Jul 16, 2014·Critical Reviews in Biotechnology·Jeong-Hun KangMasaharu Murata
Mar 14, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Y-L ZhaoX-H Xiao
Dec 15, 2006·Gene Therapy·H Herweijer, J A Wolff
Jun 19, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Evelyne ZeiraEithan Galun
Aug 9, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hsin-Lin Lu, Fang Liao

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