PMID: 11311831Apr 20, 2001Paper

The use of the LightCycler for the detection of Y chromosome SNPs

Forensic Science International : Synergy
M V LareuA Carracedo

Abstract

A novel methodology based on PCR monitoring on-line with fluorescent formats using the LightCycler for Y chromosome SNP typing is proposed. The main advantages of the system are the time necessary for the analysis (which is around 20 min), the robustness and the accuracy of the method and especially its sensitivity, which permits the detection of the male component in male-female mixtures up to 1:300 for some of the SNPs. Singleplexes of four different SNPs (M9, sY81, SRY-1532 and SRY-2627) as well as two duplexes (M9 and sY81 on the one hand and SRY-1532 and SRY-2627 on the other) were efficiently implemented. A simultaneous amplification and analysis of the four SNPs is also possible. It seems difficult with the current methodology to implement more than a quadruplex.

References

Jul 1, 1992·Forensic Science International : Synergy·F BarrosA Carracedo
Dec 1, 1994·Human Molecular Genetics·M T SeielstadL L Cavalli-Sforza
Jan 1, 1993·International Journal of Legal Medicine·E ValverdeA Carracedo
Mar 14, 1997·Forensic Science International : Synergy·M PrinzB Shaler
Feb 11, 1999·American Journal of Human Genetics·F R SantosR J Mitchell
Feb 19, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·A CarracedoF Dominguez
Jul 7, 2000·Forensic Science International : Synergy·L GusmãoA Carracedo

❮ Previous
Next ❯

Citations

Aug 28, 2009·International Journal of Legal Medicine·Marielle HeinrichUlrike Schmidt
Mar 26, 2004·Forensic Science International : Synergy·Antonio AlonsoJose Fernández-Piqueras
Mar 17, 2004·Forensic Science International : Synergy·Jon H WettonAdrian C Spriggs
Mar 24, 2004·Forensic Science International : Synergy·Juan J SanchezAngel Carracedo
Mar 24, 2004·Forensic Science International : Synergy·B QuintánsA Carracedo
Jun 27, 2002·Forensic Science International : Synergy·Jon H WettonAdrian C Spriggs
Sep 19, 2008·Journal of Forensic Sciences·Janice A Nicklas, Eric Buel
Sep 27, 2005·Forensic Science International : Synergy·Beatriz SobrinoAngel Carracedo
Sep 27, 2005·Forensic Science International : Synergy·Anna-Maria Divne, Marie Allen
Jun 21, 2007·American Journal of Clinical Pathology·Victoria SnowdonDouglas James Demetrick
Nov 9, 2004·Bone Marrow Transplantation·L W HarriesS Ellard
Aug 30, 2003·Analytical Chemistry·T A BrettellR Saferstein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.