The use of the MTT test for determining the cytotoxicity of veterinary drugs in pig hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M B Huveneers-OorsprongH A Kuiper

Abstract

Pig hepatocytes were used for determining the cytotoxicity of a number of veterinary drugs and known hepatotoxic compounds, using the MTT test as a marker for viability. When possible, drugs were tested in the absence and presence of dimethyl sulfoxide (DMSO), to study the possible effect of this solvent when used in the case of less hydrophilic compounds. IC(50) values calculated from the dose-response curves for acetylsalicylic acid (7 mm) and acetaminophen (paracetamol; 10 mm) in rat hepatocytes were similar to those reported by other groups. IC(50) values for acetylsalicylic acid (8.7 mm), erythromycin (0.7 mm), chloramphenicol (8.1 mm), stilboestrol (diethylstilboestrol; 0.16 mm and propranolol (0.17 mm) in pig hepatocytes were similar to those reported in the literature for rat hepatocytes. In comparison to rat hepatocytes, clenbuterol was about equally cytotoxic in pig hepatocytes (IC(50) of 2.1 nu. 1.6 mm), whereas paracetamol was much more cytotoxic (IC(50) of 2.8 nu. 10 mm). Unlike chloramphenicol, the related drug thiamphenicol showed no signs of decreased MTT formation in pig hepatocytes at the highest possible test concentration of 10 mm, as was the case for furazolidone, oxytetracycline, carbadox and the putative ...Continue Reading

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May 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L A HoogenboomH A Kuiper

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Citations

Jan 5, 2002·Biomaterials·Tsui-Hsien HuangChia-Tze Kao
Jan 5, 2007·Journal of Medicinal Food·J K Kumi-DiakaVanessa Horman
May 2, 2009·Journal of Tissue Engineering and Regenerative Medicine·Poonam VermaAlok R Ray
Aug 19, 2007·Journal of Biomedical Materials Research. Part a·Mingbo WangJ A Jansen
Aug 23, 2008·Journal of Applied Toxicology : JAT·María I AmaroJaime Charris
Jun 27, 2006·Chemico-biological Interactions·Chong ShenQin Meng
Jun 9, 2009·Bioorganic & Medicinal Chemistry Letters·Dong-Jo ChangYoung-Ger Suh
Jan 18, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ana VulićNevenka Kopjar
Aug 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Manuelle BongoRoisin M Owens
Mar 14, 2018·AAPS PharmSciTech·Aroonsri PripremWanwisa Limphirat

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