The use of the polymerase chain reaction to detect bacteria in amniotic fluid in pregnancies complicated by preterm labor

American Journal of Obstetrics and Gynecology
G R MarkensonM Yancey

Abstract

The purpose of this investigation was to determine the feasibility of using the polymerase chain reaction to detect bacteria in amniotic fluid and to compare pregnancy outcomes in subsets of women categorized by amniotic fluid culture, polymerase chain reaction, and interleukin-6 findings. Amniotic fluid from 54 pregnancies with preterm labor and no clinical evidence of intraamniotic infection was evaluated with use of the polymerase chain reaction, interleukin-6, and bacterial culture. Gestational age, newborn weight, and time between amniocentesis and delivery were compared between subsets of women categorized by these tests. With use of the polymerase chain reaction <100 bacteria per milliliter could be detected in amniotic fluid. A total of 55.5% of the amniotic fluid samples were polymerase chain reaction positive, whereas 9.2% of culture results were positive. Birth weights and gestational age at delivery were less and time from amniocentesis to delivery was shorter in the polymerase chain reaction-positive group (p < 0.05). Nine samples (15%) had elevated interleukin-6 concentrations; of these, six were polymerase chain reaction positive. The polymerase chain reaction is a sensitive means of detecting bacteria in amnioti...Continue Reading

References

May 1, 1992·American Journal of Obstetrics and Gynecology·R S GibbsR L Sweet
Jan 15, 1991·FEMS Microbiology Letters·H M PalmerD Taylor-Robinson
Sep 1, 1989·American Journal of Obstetrics and Gynecology·M A SkollB M Sibai
Sep 15, 1994·The New England Journal of Medicine·P HohlfeldM Vidaud

❮ Previous
Next ❯

Citations

Mar 15, 2005·Infectious Diseases in Obstetrics and Gynecology·Michele StrakaDavid Stamilio
Apr 28, 2009·Current Opinion in Infectious Diseases·Catalin S BuhimschiIrina A Buhimschi
Aug 16, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Roberto RomeroYeon Mee Kim
Dec 9, 2015·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Sun Min KimBo Hyun Yoon
Dec 6, 2011·Seminars in Fetal & Neonatal Medicine·Daniel B DiGiulio
Sep 6, 2011·Clinics in Perinatology·Jamie A BastekMichal A Elovitz
Dec 1, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Nikolaos ThomakosAris Antsaklis
Nov 16, 2004·Trends in Endocrinology and Metabolism : TEM·Michal A Elovitz, Conjeevaram Mrinalini
Aug 30, 2005·Obstetrics and Gynecology Clinics of North America·Laura L Klein, Ronald S Gibbs
Jan 15, 2014·Journal of Perinatal Medicine·Boldizsár HorvathJózsef Bódis
Mar 16, 2013·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Tamara StampalijaTinnakorn Chaiworapongsa
Oct 18, 2011·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Sun Min KimBo Hyun Yoon
Sep 14, 2013·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Roberto RomeroTinnakorn Chaiworapongsa
Apr 25, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Maria-Teresa GervasiGiorgio Palù
Feb 18, 2015·American Journal of Obstetrics and Gynecology·C Andrew CombsUNKNOWN Obstetrix Collaborative Research Network
Nov 28, 2013·American Journal of Obstetrics and Gynecology·C Andrew CombsUNKNOWN ProteoGenix/Obstetrix Collaborative Research Network
Jan 2, 2008·American Journal of Obstetrics and Gynecology·Andrew B OnderdonkUNKNOWN Extremely Low Gestational Age Newborns (ELGAN) Study Investigators
Mar 30, 2000·American Journal of Obstetrics and Gynecology·B H YoonT R Han
Jul 31, 2004·American Journal of Obstetrics and Gynecology·Laura L Klein, Ronald S Gibbs
Dec 17, 2014·Frontiers in Immunology·Matthew S Payne, Sara Bayatibojakhi
Aug 25, 2001·Paediatric and Perinatal Epidemiology·R RomeroY M Kim
Mar 29, 2002·Mental Retardation and Developmental Disabilities Research Reviews·Luís F GonçalvesRoberto Romero
Feb 24, 2006·Journal of Perinatal Medicine·Asher BashiriMoshe Mazor
Aug 8, 2018·American Journal of Reproductive Immunology : AJRI·Lisa M RogersDavid M Aronoff
Jul 18, 2006·Obstetrical & Gynecological Survey·Catalin S BuhimschiIrina A Buhimschi
Jun 1, 2005·BJOG : an International Journal of Obstetrics and Gynaecology·Bo JacobssonHenrik Hagberg
Apr 25, 2003·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·J EspinozaY Sorokin
May 21, 2015·PloS One·Marian KacerovskyJana Nekvindova
Jan 11, 2011·American Journal of Reproductive Immunology : AJRI·Camila MarconiMárcia Guimarães da Silva
Dec 28, 2017·American Journal of Reproductive Immunology : AJRI·Noriko YonedaShigeru Saito
Aug 14, 2019·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Alisa KachikisKristina M Adams Waldorf
Jan 9, 2003·Clinics in Perinatology·Rodney E Willoughby, Karin B Nelson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.