The use of tri5 gene sequences for PCR detection and taxonomy of trichothecene-producing species in the Fusarium section Sporotrichiella

International Journal of Food Microbiology
Ludwig NiessenRudi F Vogel

Abstract

Purified DNA from isolates of Fusarium poae, Fusarium sporotrichioides, Fusarium kyushuense and Fusarium langsethiae was used as a template to amplify a 658-bp fragment from the trichodiene synthase (tri5) gene of these fungi with the gene-specific PCR primer pair Tox5-1/Tox5-2. Fragments obtained were isolated and sequenced. DNA sequence alignments revealed high similarity between the sequences derived from F. sporotrichioides and F. langsethiae (98.7%) and less similarity between the latter species and F. poae (90.9%). Phylogenetic analysis of the aligned sequences using the tri5 sequence of Fusarium pseudograminearum as an outgroup revealed clear separation between one group consisting of F. poae and F. kyushuense and another consisting of F. sporotrichioides and F. langsethiae. The two latter species could not be distinguished phylogenetically on the basis of their tri5 sequences. Taxon-specific reverse primers were designed from the aligned sequences and combined with the tri5 gene-specific forward primer Tox5-1. The new reverse primers enabled specific amplification of a fragment of approximately 400 bp from DNA isolated from F. sporotrichioides, F. poae, F. langsethiae and F. kyushuense, respectively. All primers were te...Continue Reading

References

May 1, 1992·Molecular Plant-microbe Interactions : MPMI·T M Hohn, A E Desjardins
Jul 1, 1995·Molecular Plant-microbe Interactions : MPMI·R H ProctorS P McCormick
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Jun 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Todd J WardKerry O'Donnell
Oct 31, 2002·Applied and Environmental Microbiology·B BakanY Brygoo
Sep 1, 2004·International Journal of Food Microbiology·Mona Torp, Andreas Adler
Sep 1, 2004·International Journal of Food Microbiology·Mona Torp, Helgard I Nirenberg
Sep 1, 2004·International Journal of Food Microbiology·Ulf ThraneAlberto Ritieni
Sep 1, 2004·International Journal of Food Microbiology·Holger SchmidtRudi F Vogel

❮ Previous
Next ❯

Citations

Feb 23, 2012·Journal of Applied Genetics·Łukasz StępieńJerzy Chełkowski
Jan 12, 2010·Arhiv za higijenu rada i toksikologiju·Dario IvićBogdan Cvjetković
Sep 7, 2007·International Journal of Food Microbiology·Ludwig Niessen
May 22, 2007·International Journal of Food Microbiology·Markus Schmidt-Heydt, Rolf Geisen
Sep 1, 2004·International Journal of Food Microbiology·Mona Torp, Helgard I Nirenberg
Apr 28, 2012·FEMS Microbiology Letters·María I DinolfoSebastián A Stenglein
Mar 24, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Veli HietaniemiPäivi Parikka
Aug 5, 2017·Journal of Molecular Microbiology and Biotechnology·Adam KuzdralińskiMarta Muszyńska

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.