The use of whole-genome sequencing in cluster investigation of a multidrug-resistant tuberculosis outbreak

The European Respiratory Journal
Maeve K LalorIbrahim Abubakar

Abstract

We used whole-genome sequencing (WGS) to delineate transmission networks and investigate the benefits of WGS during cluster investigation.We included clustered cases of multidrug-resistant (MDR) tuberculosis (TB)/extensively drug-resistant (XDR) TB linked by mycobacterial interspersed repetitive unit variable tandem repeat (MIRU-VNTR) strain typing or epidemiological information in the national cluster B1006, notified between 2007 and 2013 in the UK. We excluded from further investigation cases whose isolates differed by greater than 12 single nucleotide polymorphisms (SNPs). Data relating to patients' social networks were collected.27 cases were investigated and 22 had WGS, eight of which (36%) were excluded as their isolates differed by more than 12 SNPs to other cases. 18 cases were ruled into the transmission network based on genomic and epidemiological information. Evidence of transmission was inconclusive in seven out of 18 cases (39%) in the transmission network following WGS and epidemiological investigation.This investigation of a drug-resistant TB cluster illustrates the opportunities and limitations of WGS in understanding transmission in a setting with a high proportion of migrant cases. The use of WGS should be com...Continue Reading

References

Feb 25, 2011·The New England Journal of Medicine·Jennifer L GardyPatrick Tang
Nov 20, 2012·The Lancet Infectious Diseases·Timothy M WalkerTim E A Peto
Mar 1, 2013·BMC Infectious Diseases·Josephine M BryantDick van Soolingen
Jun 5, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M W Borgdorff, D van Soolingen
Jul 17, 2013·Current Opinion in Critical Care·Vien Thi Minh Le, Binh An Diep
Jan 28, 2014·Nature Genetics·Nicola CasaliFrancis Drobniewski
Mar 13, 2014·Spatial and Spatio-temporal Epidemiology·Aman VermaDavid L Buckeridge
Apr 10, 2014·Molecular Biology and Evolution·Xavier DidelotCaroline Colijn
Aug 19, 2015·The Lancet Infectious Diseases·Howard E Takiff, Oscar Feo
Dec 17, 2015·The Lancet. Respiratory Medicine·Louise J PankhurstUNKNOWN COMPASS-TB Study Group
Jul 16, 2016·Thorax·Martin Dedicoat, Graham S Cooke
Mar 17, 2017·The Lancet Infectious Diseases·Timothy M WalkerDerrick W Crook
Nov 18, 2017·American Journal of Epidemiology·Colin J WorbyWilliam P Hanage

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Citations

Sep 11, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Qi JiangQian Gao
Jul 29, 2020·Journal of Clinical Microbiology·Sophie GeorgeKate E Dingle
Feb 23, 2019·Emerging Infectious Diseases·Juan DomínguezDarío García de Viedma
May 7, 2019·Frontiers in Public Health·Marieke J van der Werf, Csaba Ködmön
Feb 25, 2020·Emerging Infectious Diseases·Matthias MerkerStefan Niemann
Sep 21, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Allison WolfMax R O'Donnell
Feb 6, 2021·Clinical Medicine : Journal of the Royal College of Physicians of London·James BrownMarc Lipman
Mar 6, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Prince AsareDorothy Yeboah-Manu
Aug 10, 2021·Frontiers in Public Health·Prince AsareDorothy Yeboah-Manu

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