The objective was to determine if a single measurement of plasma insulin-like growth factor-1 (IGF-1) could predict the number of viable embryos obtained from donors and the likelihood of pregnancy in recipients in multiple ovulation and embryo transfer (MOET) programs in cattle. The embryo yields from 101 embryo recoveries were examined in maiden Holstein heifers (n=75) and multiparous Holstein cows (lactating cows n=20, dry cows n=6). Donors were super stimulated with FSH and embryo recovery was done non-surgically 7 days after artificial insemination. Embryos were classified according to the IETS criteria. Pregnancy rates in 100 maiden Holstein heifer recipients were analysed. Recipients were on day 7+/-1 of the estrous cycle at transfer. Pregnancy diagnosis was carried out at day 30 (PD 30) and rechecked at day 60 (PD 60) after transfer. Blood samples from coccygeal vessels taken at the time of embryo recovery (donors) and transfer (recipients) were analysed for IGF-1, insulin, beta-hydroxybutyrate (beta-OHB), non-esterified fatty acids (NEFA), urea and cholesterol. There was a negative correlation between the number of viable embryos and insulin (r=-0.33, P=0.025) in donor heifers. In donor cows, the number of viable embry...Continue Reading
Binding and action of insulin-like growth factors and insulin in bovine luteal tissue during the oestrous cycle
Tissue concentration, mRNA expression and stimulation of IGF-I in luteal tissue during the oestrous cycle and pregnancy of cows
Concentrations of insulin-like growth factor-I in blood and ovarian follicular fluid of cattle selected for twins
Effect of exogenous insulin on plasma and follicular insulin-like growth factor I, insulin-like growth factor binding protein activity, follicular oestradiol and progesterone, and follicular growth in superovulated Angus and Brahman cows
Effects of somatotropin on the conceptus, uterus, and ovary during maternal recognition of pregnancy in cattle
Relationships between the number of small follicles prior to superovulatory treatment and superovulatory response in Holstein cows
Assessment of the presence of a dominant follicle and selection of dairy cows suitable for superovulation by a single ultrasound examination
Role for insulin-like growth factor I in the regulation of electrolyte composition of uterine luminal fluid
Expression of growth factor ligand and their receptor mRNAs in bovine ova during in vitro maturation and after fertilization in vitro
Superovulatory response of one ovary is related to the micro- and macroscopic population of follicles in the contralateral ovary of the Cow
Liver-derived insulin-like growth factor I (IGF-I) is the principal source of IGF-I in blood but is not required for postnatal body growth in mice
Expression of the insulin-like growth factor-1 gene and its receptor in preimplantation mouse embryos; is it a marker of embryo viability?
Follicular and luteal expression of insulin-like growth factors I and II and the type 1 IGF receptor in the bovine ovary
The expression of the IGF system in the bovine uterus throughout the oestrous cycle and early pregnancy
Culture of bovine preantral follicles in a serum-free system: markers for assessment of growth and development
Effect of nutrition and superovulation on oocyte morphology, follicular fluid composition and systemic hormone concentrations in ewes
Relationship between time of first cleavage and the expression of IGF-I growth factor, its receptor, and two housekeeping genes in bovine two-cell embryos and blastocysts produced in vitro
Expression of mRNA encoding insulin-like growth factors I and II and the type 1 IGF receptor in the bovine corpus luteum at defined stages of the oestrous cycle
Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows.
Effect of long-term treatment with recombinant bovine somatotropin and estradiol on hormone concentrations and ovulatory response of superovulated cattle
Novel demonstration of a physiologic/pharmacologic role of insulin-like growth factor-1 in ovulation in rats and action on cumulus oophorus
Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle
Changes in the relative abundance of mRNA transcripts for insulin-like growth factor (IGF-I and IGF-II) ligands and their receptors (IGF-IR/IGF-IIR) in preimplantation bovine embryos derived from different in vitro systems
Prediction of the individual follicle-stimulating hormone threshold for gonadotropin induction of ovulation in normogonadotropic anovulatory infertility: an approach to increase safety and efficiency
Apoptosis and cell proliferation potential of bovine embryos stimulated with insulin-like growth factor I during in vitro maturation and culture
Preimplantation exposure to high insulin-like growth factor I concentrations results in increased resorption rates in vivo
Expression and localization of IGF family members in bovine antral follicles during final growth and in luteal tissue during different stages of estrous cycle and pregnancy
Recent discoveries in physiology of insulin-like growth factor-1 and its interaction with gonadotropins in folliculogenesis
Effects of growth hormone and insulin-like growth factor-I on development of in vitro derived bovine embryos
Bovine somatotropin increases embryonic development in superovulated cows and improves post-transfer pregnancy rates when given to lactating recipient cows
Insulin-like growth factor (IGF) system in the oocyte and somatic cells of bovine preantral follicles
Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy
Regulation of apoptosis in the bovine blastocyst by insulin and the insulin-like growth factor (IGF) superfamily
FSH response-dose can be predicted in ovulation induction for normogonadotropic anovulatory infertility
Plasma progesterone profiles and factors affecting embryo-fetal mortality following embryo transfer in dairy cattle
Propionibacteria fed to dairy cows: effects on energy balance, plasma metabolites and hormones, and reproduction
Insulin-like growth factor-I as a possible hormonal mediator of nutritional regulation of reproduction in cattle
Growth, development, and gene expression by in vivo- and in vitro-produced day 7 and 16 bovine embryos
Comparison of stereoscopy, light microscopy and ultrastructural methods for evaluation of bovine embryos
Global gene expression in endometrium of high and low fertility heifers during the mid-luteal phase of the estrous cycle
Effects in cattle of genetic variation within the IGF1R gene on the superovulation performance and pregnancy rates after embryo transfer
The effect of polymorphism in gene of insulin-like growth factor-I on the serum periparturient concentration in Holstein dairy cows
Embryo production in superovulated goats treated with insulin before or after mating or by continuous propylene glycol supplementation
Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part I. The importance of negative energy balance and altered corpus luteum function to the reduction of oocyte and embryo quality in high-yielding dairy cows
Impact of maternal malnutrition during the periconceptional period on mammalian preimplantation embryo development
Characterization of the endometrial transcriptome in early diestrus influencing pregnancy status in dairy cattle after transfer of in vitro-produced embryos.
Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep.
Metabolite Profiling in the Pursuit of Biomarkers for IVF Outcome: The Case for Metabolomics Studies
Genome-wide scan highlights the role of candidate genes on phenotypic plasticity for age at first calving in Nellore heifers.
Effect of insulin or insulin-like growth factor-I administration at mid-luteal phase of the estrous cycle during superovulation on hormonal profile of Sahiwal cows
Metabolic biomarkers, body condition, uterine inflammation and response to superovulation in lactating Holstein cows.
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