The usefulness of amplified fragment length polymorphism markers for taxon discrimination across graduated fine evolutionary levels in Caribbean Anolis lizards

Molecular Ecology
R Ogden, R S Thorpe

Abstract

Fine-level taxon discrimination is important in biodiversity assessment and ecogeographical research. Genomic markers are often required for studies on closely related taxa, however, most existing mitochondrial and nuclear markers require prior knowledge of the genome and are impractical for use in small conservation projects. This study describes the application of amplified fragment length polymorphism (AFLP) to discriminate at four progressively finer evolutionary levels of Caribbean Anolis lizards from the central Lesser Antilles. AFLP is shown to be a rapid and effective method for discriminating between species. Separation increases with primer pair number and choice of primer combination appears to be noncritical. Initial population-level results show markedly less discriminatory power. A screening technique for the identification of population informative markers combining principal component and principal coordinate analyses is presented and assessed. Subsequent results show selected conspecific AFLP data to be remarkably congruent with those of mitochondrial DNA, microsatellite and morphological markers. The use of AFLP as a low-cost nuclear marker in species-level taxon discrimination is supported, whereas population...Continue Reading

References

Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·R C AlbertsonT D Kocher
Sep 11, 1999·Trends in Ecology & Evolution·U G Mueller, L L Wolfenbarger
Apr 27, 2000·Animal Genetics·C OviloJ M Martínez-Zapater
Jan 4, 2001·Journal of Molecular Evolution· de Knijff PUNKNOWN Port of Rotterdam Gull Study Group
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rob Ogden, Roger S Thorpe
Oct 1, 1999·Evolution; International Journal of Organic Evolution·Oliver R W Pergams, Mary V Ashley

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Citations

May 4, 2004·Molecular Phylogenetics and Evolution·Alexandre RiberonP Taberlet
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Charlotte J AllenderNorman Maclean
Jan 26, 2007·Proceedings. Biological Sciences·Emmanuel MilotLouis Bernatchez
Aug 17, 2005·Molecular Ecology·Staffan Bensch, Mikael Akesson
Jul 15, 2015·Molecular Phylogenetics and Evolution·MrinaliniAnita Malhotra
Sep 24, 2013·Molecular Phylogenetics and Evolution·Paul C KirchbergerStephan Koblmüller
Sep 3, 2011·The Journal of Heredity·Felipe S BarretoMichael A McCartney

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