Abstract
To investigate the uveitogenic potential of retinal S-antigen (S-Ag) in horses. Horses were immunized subcutaneously with S-Ag or BSA as control antigen, emulsified in complete Freund's adjuvant. Simultaneously, Bordetella pertussis was given intravenously. Antigen specific T- and B-cell responses were analyzed in a 3-day interval. Disease development was judged clinically and histopathologically. Two identical booster immunizations were given every 4 weeks to test induction of recurrences. T- and B-cell responses specific for S-Ag were observed in all immunized horses but were absent in control animals. However, uveitis developed in only one of five animals. Reimmunization with S-Ag did not lead to a uveitic relapse in this horse. All other horses of the S-Ag- and BSA-treated groups neither showed any signs of uveitis, nor had inflammatory infiltrates of the inner eye. In contrast to interphotoreceptor retinoid-binding protein (IRBP), S-Ag is a weak autoantigen in horses. Even though S-Ag immunization leads to the activation of autoreactive T- and B-cells, infiltration of the inner eye and induction of uveitis are controlled in most horses.
Citations
Jan 22, 2008·International Immunology·Maria Diedrichs-MöhringGerhild Wildner
Mar 13, 2014·PloS One·Roxane L DegrooteCornelia A Deeg
Dec 4, 2012·International Journal of Molecular Sciences·Christoph M SzoberCornelia A Deeg
Feb 22, 2012·Expert Opinion on Biological Therapy·Juan Javier ServatJohn J Huang
Jul 8, 2014·Journal of Proteomics·P B UhlC A Deeg
Mar 26, 2010·Immunobiology·Johanna K ZippliesCornelia A Deeg
Oct 3, 2008·The Veterinary Journal·Ana M TerrasaGustavo Zapata
Apr 15, 2008·Veterinary Immunology and Immunopathology·Barbara K BrausCornelia A Deeg
Dec 17, 2008·Veterinary Ophthalmology·Cornelia A Deeg
Mar 22, 2012·Veterinary Ophthalmology·Johanna K ZippliesCornelia A Deeg
Jul 21, 2015·The Veterinary Journal·Fernando MalalanaCatherine McGowan
Feb 7, 2012·Journal of Proteomics·Roxane L DegrooteCornelia A Deeg
May 29, 2012·Journal of Proteomics·Stefanie M HauckCornelia A Deeg
Nov 26, 2008·Veterinary Immunology and Immunopathology·Cornelia A Deeg
Apr 25, 2016·Journal of Neuroinflammation·Cornelia A DeegStefanie M Hauck
Jun 28, 2016·Veterinary Research Communications·Galina P SimeonovaRadostin S Simeonov
Sep 26, 2013·Journal of Proteome Research·Juliane MerlStefanie M Hauck
Oct 23, 2013·Journal of Proteome Research·Roxane L DegrooteCornelia A Deeg
May 13, 2006·Molecular & Cellular Proteomics : MCP·Cornelia A DeegMarius Ueffing
Apr 5, 2007·Proteomics·Cornelia A DeegMarius Ueffing
Aug 19, 2018·Scientific Reports·Melanie SchauerCornelia A Deeg
May 21, 2019·Frontiers in Pharmacology·Jatin Sharma, Joseph Larkin
Mar 27, 2020·Frontiers in Cell and Developmental Biology·Carmen WiedemannCornelia A Deeg
Dec 31, 2008·Journal of Proteome Research·Johanna K ZippliesCornelia A Deeg