The uvsY recombination protein of bacteriophage T4 forms hexamers in the presence and absence of single-stranded DNA

Biochemistry
Hans T H Beernink, Scott W Morrical

Abstract

A prerequisite to genetic recombination in the T4 bacteriophage is the formation of the presynaptic filament-a helical nucleoprotein filament containing stoichiometric amounts of the uvsX recombinase in complex with single-stranded DNA (ssDNA). Once formed, the filament is competent to catalyze homologous pairing and DNA strand exchange reactions. An important component in the formation of the presynaptic filament is the uvsY protein, which is required for optimal uvsX-ssDNA assembly in vitro, and essential for phage recombination in vivo. uvsY enhances uvsX activities by promoting filament formation and stabilizing filaments under conditions of low uvsX, high salt, and/or high gp32 (ssDNA-binding protein) concentrations. The molecular properties of uvsY include noncooperative binding to ssDNA and specific protein-protein interactions with both uvsX and gp32. Evidence suggests that all of these hetero-associations of the uvsY protein are important for presynaptic filament formation. However, there is currently no structural information available on the uvsY protein itself. In this study, we present the first characterization of the self-association of uvsY. Using hydrodynamic methods, we demonstrate that uvsY associates into a ...Continue Reading

References

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Citations

Sep 29, 1999·Trends in Biochemical Sciences·H T Beernink, S W Morrical
Feb 27, 2009·Nucleic Acids Research·Joshua N Farb, Scott W Morrical
Oct 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hang XuScott W Morrical
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Dec 7, 2010·Virology Journal·Jie Liu, Scott W Morrical
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·G MosigD Vo
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·J S BleuitS W Morrical
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·D A Fancy, T Kodadek
Jun 21, 2008·Journal of Molecular Biology·Kiran PantMark C Williams
Mar 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Stefan GajewskiStephen W White
Apr 6, 2004·Structure·E Allen SickmierStephen W White
Apr 8, 2000·Trends in Biochemical Sciences·K N Kreuzer
May 24, 2011·Critical Reviews in Biochemistry and Molecular Biology·Jie LiuScott W Morrical

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