PMID: 9642196Jun 27, 1998Paper

The V antigen of Yersinia pestis regulates Yop vectorial targeting as well as Yop secretion through effects on YopB and LcrG

Journal of Bacteriology
Matthew L NillesS C Straley

Abstract

Yersinia pestis expresses a set of secreted proteins called Yops and the bifunctional LcrV, which has both regulatory and antihost functions. Yops and LcrV expression and the activity of the type III mechanism for their secretion are coordinately regulated by environmental signals such as Ca2+ concentration and eukaryotic cell contact. In vitro, Yops and LcrV are secreted into the culture medium in the absence of Ca2+ as part of the low-Ca2+ response (LCR). The LCR is induced in a tissue culture model by contact with eukaryotic cells that results in Yop translocation into cells and subsequent cytotoxicity. The secretion mechanism is believed to indirectly regulate expression of lcrV and yop operons by controlling the intracellular concentration of a secreted negative regulator. LcrG, a secretion-regulatory protein, is thought to block secretion of Yops and LcrV, possibly at the inner face of the inner membrane. A recent model proposes that when the LCR is induced, the increased expression of LcrV yields an excess of LcrV relative to LcrG, and this is sufficient for LcrV to bind LcrG and unblock secretion. To test this LcrG titration model, LcrG and LcrV were expressed alone or together in a newly constructed lcrG deletion strai...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·D HanahanF R Bloom
Aug 1, 1980·Journal of Bacteriology·B R BochnerB N Ames
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·P WattiauG R Cornelis
Dec 1, 1994·Infection and Immunity·J M Fowler, R R Brubaker
Jan 1, 1997·Clinical Microbiology Reviews·R D Perry, J D Fetherston
Mar 1, 1997·Infection and Immunity·J Nemeth, S C Straley
Mar 1, 1997·Molecular Microbiology·G R Cornelis, H Wolf-Watz
Mar 15, 1997·The Journal of Clinical Investigation·M FällmanH Wolf-Watz
Mar 12, 1998·Journal of Bacteriology·M R SarkerG R Cornelis

❮ Previous
Next ❯

Citations

Aug 11, 2005·Molecular Microbiology·Franco FerracciGregory V Plano
Nov 21, 2007·Infection and Immunity·Stanislav FormanSusan C Straley
Mar 20, 2014·Microbiology and Immunology·Teiji SawaHiroaki Yasumoto
Aug 8, 2001·Journal of Bacteriology·J S Matson, M L Nilles
Apr 9, 2001·Infection and Immunity·R Nordfelth, H Wolf-Watz
Apr 20, 2001·Molecular Microbiology·G V PlanoF Ferracci
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Guy R Cornelis
Jul 24, 2007·Journal of Bacteriology·Mohamad A Hamad, Matthew L Nilles
Dec 12, 2012·Journal of Bacteriology·Katherine Given LigtenbergOlaf Schneewind
Feb 13, 1999·Molecular Microbiology·C Neyt, G R Cornelis
Aug 24, 2011·Infection and Immunity·Nathaniel D LambertDavid S Bradley
Feb 13, 2001·Molecular Microbiology·A HolmströmA Forsberg
Jul 8, 2009·Infection and Immunity·Zhan YeSusan C Straley
Oct 29, 1998·Journal of Bacteriology·G R Cornelis
Apr 28, 1999·Journal of Bacteriology·P L Payne, S C Straley
Sep 25, 1999·Infection and Immunity·K A FieldsS C Straley
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·G R CornelisI Stainier
Mar 14, 2002·International Journal of Medical Microbiology : IJMM·Guy R Cornelis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.