PMID: 9546659Apr 18, 1998Paper

The V3 loop of human immunodeficiency virus type-1 envelope protein is a high-affinity ligand for immunophilins present in human blood

European Journal of Biochemistry
M M Endrich, H Gehring

Abstract

Human immunodeficiency virus type-1 (HIV-1) infection requires binding of the envelope protein gp120 to host CD4 receptors and the action of the chemokine receptors CXCR4 or CCR5, which define cell tropism. The proline-containing V3 loop of gp120 determines the selection of the chemokine receptor and participates in conformational changes on binding of gp120 to CD4. In this study, we show that macrophage-tropic and T-cell-tropic V3 loop peptides bind specifically to the active site of the immunophilins FK506-binding protein (FKBP12), and cyclophilins A and B. Macrophage-tropic and T-cell-tropic V3 loop peptides inhibited the peptidyl-prolyl cis-trans isomerase (PPIase) activities of the immunophilins. Kd values in the range 0.036-4.1 microM were determined with V3 loop peptides labeled with an environmentally sensitive fluorophore. The observed binding properties of the V3 loop peptides reveal structural motifs of linear water-soluble peptidic substrates for tight interaction with immunophilins. FKBP12, and cyclophilins A and B were found to be present in normal human blood in the ranges 0.8-1.7, 1.4-2.3 and 2.4-3.1 nM, respectively, as demonstrated by PPIase activity measurements and western blot analysis. Cyclophilins A and B...Continue Reading

Citations

Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Joseph P SteinerAvindra Nath
Nov 2, 2013·Cell Death & Disease·P NigroM C Capogrossi
Jan 1, 2000·Journal of Biomolecular Structure & Dynamics·V Tugarinov, J Anglister
Dec 26, 2008·Journal of Biomolecular Structure & Dynamics·Alexander M Andrianov
Jul 9, 2009·Journal of Biomolecular Structure & Dynamics·Alexander M Andrianov, Ivan V Anishchenko
May 14, 2005·Journal of Virology·Réjean CantinMichel J Tremblay
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Fabrice AllainGeneviève Spik
Dec 1, 2001·Expert Opinion on Biological Therapy·B G Brenner, Z Wainberg
Feb 26, 2008·Veterinary Immunology and Immunopathology·Sean P TrothEdward A Hoover
Dec 8, 2004·Journal of Neuroimmunology·C L AchimM Avramut
Oct 31, 2000·Circulation Research·Z G JinB C Berk
Feb 10, 1999·The American Journal of Physiology·M P MerkerC A Dawson
Jul 10, 2003·Chemical Reviews·Christophe Dugave, Luc Demange

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