The vacuole/lysosome is required for cell-cycle progression

ELife
Yui Jin, Lois S Weisman

Abstract

Organelles are distributed to daughter cells, via inheritance pathways. However, it is unclear whether there are mechanisms beyond inheritance, which ensure that organelles are present in all cells. Here we present the unexpected finding that the yeast vacuole plays a positive essential role in initiation of the cell-cycle. When inheritance fails, a new vacuole is generated. We show that this occurs prior to the next cell-cycle, and gain insight into this alternative pathway. Moreover, we find that a combination of a defect in inheritance with an acute block in the vacuole biogenesis results in the loss of a functional vacuole and a specific arrest of cells in early G1 phase. Furthermore, this role for the vacuole in cell-cycle progression requires an intact TORC1-SCH9 pathway that can only signal from a mature vacuole. These mechanisms may serve as a checkpoint for the presence of the vacuole/lysosome.

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Citations

May 7, 2016·Current Biology : CB·Yee-Hung M ChanWallace F Marshall
Apr 30, 2016·Current Opinion in Cell Biology·Barbara Knoblach, Richard A Rachubinski
Apr 22, 2018·Journal of Cell Science·Sarah OuahoudDaniel F Markgraf
Jun 2, 2018·Journal of Cell Science·Yuko SukegawaYoshikazu Ohya
Sep 20, 2019·Current Genetics·Riddhi BanerjeeShirisha Nagotu
Sep 1, 2017·Current Genetics·Elja EskesJoris Winderickx
Sep 26, 2017·Cellular Logistics·Addison RainsMelanie L Styers
Dec 15, 2017·Molecular Biology of the Cell·Eigo TakedaAkira Matsuura
Jul 11, 2020·Physical Biology·William ChadwickYee-Hung Mark Chan
Aug 10, 2019·Cancer Research·Laia Martínez-CarreresLluis Fajas
Feb 6, 2021·Advances in Biological Regulation·Sara Wong, Lois S Weisman
Oct 10, 2020·Physical Biology·William L ChadwickYee-Hung M Chan
May 6, 2021·The Journal of Cell Biology·Chonglin Yang, Xiaochen Wang
Jun 3, 2021·Cells·Bryce OrdwayMehdi Damaghi

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Methods Mentioned

BETA
dissection
dissections
flow cytometry
FACS
PCR

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