Abstract
The aetiology of bacterial vaginosis (BV) is not well-understood, and prevalence appears to be higher among women living in sub-Saharan Africa. A recent conceptual model implicates three main bacteria (Gardnerella vaginalis; Atopobium vaginae; and Prevotella bivia), sexual activity, sialidase activity, and biofilm formation in the pathogenesis of BV. We describe the vaginal microbiota, presence of the putative sialidase A gene of G. vaginalis, and biofilm among 386 adolescent girls aged 17 and 18 years in a cross-sectional study in Mwanza, Tanzania around the time of expected sexual debut. Vaginal swabs were collected and tested by quantitative polymerase chain reaction (qPCR) for five Lactobacillus species, G. vaginalis, A. vaginae, P. bivia, the sialidase A gene of G. vaginalis, and by fluorescence in situ hybridisation (FISH) for evidence of G. vaginalis and A. vaginae biofilm. We conducted a risk factor analysis of G. vaginalis, A. vaginae and P. bivia, and explored the associations between biofilm, the presence of the sialidase A gene, and non-optimal vaginal microbiota (Nugent 4-7). L. crispatus and L. iners were detected in 69 and 82% of girls, respectively. The prevalence of L. crispatus was higher than previously repor...Continue Reading
References
Feb 1, 1991·Journal of Clinical Microbiology·R P NugentS L Hillier
Nov 29, 2007·The ISME Journal·Xia ZhouLarry J Forney
Feb 20, 2008·Sexually Transmitted Infections·C-Y ChenR C Ballard
Oct 25, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Katherine A FethersCatriona S Bradshaw
Nov 4, 2008·American Journal of Obstetrics and Gynecology·Ronald H GrayMaria J Wawer
Apr 1, 2010·BMC Infectious Diseases·Hans VerstraelenMarleen Temmerman
Apr 23, 2010·Tropical Medicine & International Health : TM & IH·Lisa F LanghaugFrances M Cowan
Jun 5, 2010·Sexually Transmitted Infections·M J HopkinsI J Hart
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jacques RavelLarry J Forney
Jul 29, 2010·AIDS and Behavior·Caroline F AllenRichard J Hayes
Mar 2, 2011·PLoS Medicine·Nicola LowAdriane Martin Hilber
Mar 30, 2011·American Journal of Obstetrics and Gynecology·Guido Lopes dos Santos SantiagoMario Vaneechoutte
Mar 1, 2012·PloS One·Katherine FethersCatriona S Bradshaw
Jun 1, 2012·BMC Microbiology·Vicky JespersTania Crucitti
Oct 3, 2012·PloS One·Guido Lopes dos Santos SantiagoMario Vaneechoutte
Nov 22, 2012·Obstetrics and Gynecology·Caroline M MitchellLaura Koutsky
May 11, 2013·American Journal of Obstetrics and Gynecology·Chris KenyonTania Crucitti
Apr 18, 2014·The Journal of Infectious Diseases·Catherine F HoulihanDeborah Watson-Jones
Jul 31, 2014·Microbiology·Jennifer M FettweisGregory A Buck
Aug 26, 2014·PloS One·Janneke H H M van de WijgertVicky Jespers
Nov 13, 2014·AIDS·Anne BuveRaina N Fichorova
Apr 17, 2015·BMC Infectious Diseases·Vicky JespersUNKNOWN Vaginal Biomarkers Study Group
Aug 26, 2015·PloS One·Liselotte HardyTania Crucitti
Mar 12, 2016·Sexually Transmitted Infections·Liselotte HardyTania Crucitti
Jun 9, 2016·Obstetrics and Gynecology·Vicky JespersTania Crucitti
Jan 15, 2017·Immunity·Christina GosmannDouglas S Kwon
Feb 25, 2017·Research in Microbiology·Liselotte HardyTania Crucitti
Feb 28, 2017·PloS One·Liselotte HardyTania Crucitti
Jul 13, 2017·PloS One·Hanneke BorgdorffJanneke H H M van de Wijgert
Feb 10, 2018·AIDS·McKenna C Eastment, R Scott McClelland
Jul 13, 2018·PloS One·Migle JanulaitieneMilda Pleckaityte
Jul 24, 2018·PloS One·Tania CrucittiUNKNOWN Ring Plus study group
Dec 7, 2018·Sexually Transmitted Infections·Suzanna C FrancisAnne Buvé
Jan 15, 2019·AIDS Research and Human Retroviruses·Lyle R McKinnonGilda Tachedjian
Jan 17, 2019·International Journal of Systematic and Evolutionary Microbiology·Mario VaneechouttePiet Cools
Jan 24, 2019·The ISME Journal·Joana CastroNuno Cerca
Aug 2, 2019·The Journal of Infectious Diseases·Christina A MuznyJane R Schwebke
Nov 8, 2019·FEMS Microbiology Reviews·Aliona S RoscaNuno Cerca