The validation of a 15 STR multiplex PCR for Cannabis species.

International Journal of Legal Medicine
Stephan KöhnemannHeidi Pfeiffer

Abstract

Trade and acquisition of Cannabis drugs are illegal in many countries worldwide; nevertheless, crimes related with these drugs are a major problem for the investigative authorities. With this manuscript, we want to introduce a 15 short tandem repeat (STR) Cannabis marker set that can be amplified in one PCR reaction. This multiplex PCR is specific to Cannabis species and combines highly informative STR markers. The 15 STR multiplex is easy to use and was validated according to common laboratory quality standards. Due to the fact that a lot of Cannabis plants are cultivated by clonal propagation and may show aneuploidy, polyploidy or multiple gene loci, it is not possible to apply biostatistics that follow the Hardy-Weinberg law. However, this multiplex will help the police to trace back trade routes of drug syndicates or dealers and it can help to link Cannabis plants to a crime scene.

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Citations

Jan 7, 2014·Investigative Genetics·Hilde NybomBjörn Rotter
Dec 15, 2015·International Journal of Legal Medicine·Rachel HoustonDavid Gangitano
Sep 1, 2014·Forensic Science International. Genetics·Laura ValverdeStephan Köhnemann
Feb 18, 2014·Forensic Science International. Genetics·Laura ValverdeStephan Köhnemann
Apr 4, 2015·Disease-a-month : DM·Donald E GreydanusCynthia L Feucht
Feb 22, 2018·International Journal of Legal Medicine·Rachel HoustonDavid Gangitano
Aug 25, 2019·International Journal of Legal Medicine·Madeline G RomanRachel Houston
Jun 26, 2020·International Journal of Legal Medicine·Lucas de Oliveira Pereira RibeiroClarice Sampaio Alho
Jan 21, 2017·PloS One·Christophe DufresnesLuca Fumagalli
Oct 17, 2018·International Journal of Legal Medicine·Mauro Sander FettFlávio Anastácio de Oliveira Camargo

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