The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle

F1000Research
Gholib GholibBambang Purwantara

Abstract

Background: To obtain accurate measurements of cortisol (C) and testosterone (T) in Aceh cattle, commercial enzyme-linked immunosorbent assay (ELISA) kits need to be carefully validated. Moreover, repeated freeze-thaw cycles during the storage of the samples may affect the stability of the hormones in the serum. Here, the reliability of C and T concentration measurements in the serum of Aceh cattle, was tested using commercial C and T ELISA kits designed to measure human C and T concentrations. Further, the effect of repeated freeze-thaw cycles on the stability of C and T concentrations in the serum was evaluated. Methods: Commercial C (Cat. no. EIA-1887) and T (Cat. no. EIA-1559) ELISA kits from DRG Instruments GmbH were validated through an analytical validation test (i.e., parallelism, accuracy, and precision) and a biological validation test (for C: effect of transportation on the C secretion; for T: the concentrations of T between bulls and cows). To test the effects of freeze-thaw cycles, cattle serum was subjected to the following treatments: (i) remained frozen at -20 OC (control group); (ii) exposed to freeze-thaw cycles for two, four, six, and eight times (test groups).   Results: Parallelism, accuracy, and precision ...Continue Reading

References

Jan 19, 2000·Zentralblatt für Veterinärmedizin. Reihe A·E MöstlR Palme
Jul 27, 2002·Domestic Animal Endocrinology·E Möstl, R Palme
Aug 5, 2005·Cell and Tissue Research·Matthew P HardyChantal Sottas
Oct 20, 2007·Tropical Animal Health and Production·P H BayemiE C Webb
Jul 14, 2010·American Journal of Primatology·David J PappanoJacinta C Beehner
Feb 24, 2011·Oecologia·Michael J SheriffRudy Boonstra
Aug 31, 2013·Domestic Animal Endocrinology·M Z TahirS Chastant-Maillard
Jan 1, 2012·Molecular Metabolism·Maximilian BielohubyMartin Bidlingmaier
Jun 19, 2014·Primates; Journal of Primatology·Ariadna Rangel-NegrínPedro Américo D Dias
Jun 16, 2016·Annals of Clinical Biochemistry·J J HillebrandE Endert
Oct 25, 2016·Primates; Journal of Primatology·Taufiq Purna NugrahaTony Weingrill
Jul 29, 2017·Scientific Reports·Anne Marie Kinn RødRobert Murison
Nov 23, 2017·Journal of Natural Medicines·Seiichi SakamotoSatoshi Morimoto

❮ Previous
Next ❯

Citations

Jan 9, 2021·Journal of Veterinary Internal Medicine·Hannah M KinsellaRamiro E Toribio

❮ Previous
Next ❯

Software Mentioned

Microplate Manager
SigmaPlot
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.