The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma

Oncotarget
Can LuDeSheng Xiao

Abstract

To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements. A total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined. Immunoglobulin gene rearrangements were found in all 209 cases, with 93 IGHA, 122 IGHB, 98 IGHC, 167 IGK, 100 IGL, 167 IGHA/B/C, 204 IGH/IGK, 209 IGH/IGK/IGL, 129 IGH+IGK, 81 IGH+IGL, 83 IGK+IGL and 68 IGH+IGK+IGL gene rearrangements. Immunoglobulin gene rearrangements were not found in the 35 cases of reactive lymphoid hyperplasia. IGH and IGK gene rearrangements were mainly found in mantle cell lymphomas, small lymphocytic lymphomas, extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid t...Continue Reading

References

May 1, 1990·Journal of Clinical Pathology·K P McCarthyL M Wiedemann
Dec 4, 2002·Nature Reviews. Immunology·Hiroaki Niiro, Edward A Clark
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marko Radic
Oct 14, 2008·Current Opinion in Immunology·Eric Meffre, Hedda Wardemann
Jul 8, 2009·The Journal of Experimental Medicine·Emily J CaderaMark S Schlissel
May 22, 2013·Journal of Pediatric Hematology/oncology·Jimmy T NguyenRicarchito Manera
Apr 5, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Cong-Wei JiaZhao-Hui Lu
Dec 17, 2014·International Journal of Oncology·Ira KokovicSrdjan Novakovic
Dec 31, 2014·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Behzad PoopakNajmaldin Saki
Feb 6, 2016·Oncotarget·Seung Eun LeeYoung Hyeh Ko
Jun 30, 2016·Genes, Chromosomes & Cancer·Claudia OttoUNKNOWN “Molecular Mechanisms in Malignant Lymphomas” Network Project
Jul 28, 2016·Journal of Cancer Research and Therapeutics·Jian BoYu Zhao
Dec 4, 2016·Hematology·Premal Lulla, Helen E Heslop
Jan 11, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marek SinkoraKaterina Stepanova

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Citations

Feb 9, 2020·Journal of Clinical Pathology·Norris Hollie, Saja Asakrah
Jan 19, 2019·Oncology Letters·Naoya IshibashiToshiya Maebayashi

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Methods Mentioned

BETA
PCR
Assay
electrophoresis

Software Mentioned

IGKA
BIOMED
OLIGO

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