The vapB-vapC Operon of Acidovorax citrulli Functions as a Bona-fide Toxin-Antitoxin Module

Frontiers in Microbiology
Reut ShavitYael Helman

Abstract

Toxin-antitoxin systems are commonly found on plasmids and chromosomes of bacteria and archaea. These systems appear as biscystronic genes encoding a stable toxin and a labile antitoxin, which protects the cells from the toxin's activity. Under specific, mostly stressful conditions, the unstable antitoxin is degraded, the toxin becomes active and growth is arrested. Using genome analysis we identified a putative toxin-antitoxin encoding system in the genome of the plant pathogen Acidovorax citrulli. The system is homologous to vapB-vapC systems from other bacterial species. PCR and phylogenetic analyses suggested that this locus is unique to group II strains of A. citrulli. Using biochemical and molecular analyses we show that A. citrulli VapBC module is a bona-fide toxin-antitoxin module in which VapC is a toxin with ribonuclease activity that can be counteracted by its cognate VapB antitoxin. We further show that transcription of the A. citrulli vapBC locus is induced by amino acid starvation, chloramphenicol and during plant infection. Due to the possible role of TA systems in both virulence and dormancy of human pathogenic bacteria, studies of these systems are gaining a lot of attention. Conversely, studies characterizing ...Continue Reading

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K GerdesS Molin
Jun 1, 1971·Journal of Bacteriology·T Tosa, L I Pizer
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 1, 1994·Genetica·P AlifanoM S Carlomagno
Feb 10, 1995·Science·M B Yarmolinsky
Nov 5, 1999·Annual Review of Microbiology·H Engelberg-Kulka, G Glaser
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·T F Cooper, J A Heinemann
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·S K ChristensenK Gerdes
Oct 1, 1996·The Plant Cell·J. R. Alfano, A. Collmer
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
May 20, 2004·Journal of Bacteriology·Ronen HazanHanna Engelberg-Kulka
May 3, 2005·Nature Reviews. Microbiology·Kenn GerdesAnders Løbner-Olesen
Jul 5, 2005·Trends in Microbiology·Vickery L ArcusSusan J Turner
Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Monica BodogaiIlona Dusha
Mar 21, 2007·Molecular Microbiology·Silvia SzekeresDean A Rowe-Magnus
May 15, 2007·Journal of Bacteriology·Dayle A DainesSarah Y Yuan
May 22, 2007·Journal of Bacteriology·Virginie TsilibarisLaurence Van Melderen
Jul 10, 2007·Journal of Bacteriology·Roy David Magnuson
Apr 29, 2008·Journal of Bacteriology·Manuel Saavedra De BastLaurence Van Melderen
Jan 16, 2009·Biochemical Society Transactions·Charlotte R CooperRobert M Kelly
Jul 11, 2009·Molecular Plant-microbe Interactions : MPMI·Ofir BaharSaul Burdman
Jul 28, 2010·FEMS Microbiology Reviews·Cecília M ArraianoSandra C Viegas
Nov 3, 2010·Protein Engineering, Design & Selection : PEDS·Vickery L ArcusGregory M Cook
Apr 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kristoffer S Winther, Kenn Gerdes
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Etienne MaisonneuveKenn Gerdes
Jan 31, 2012·Nucleic Acids Research·Kristoffer S Winther, Kenn Gerdes
Mar 1, 2012·Journal of Bacteriology·Joanna L McKenzieGregory M Cook
Mar 27, 2012·Trends in Microbiology·Julia J Williams, Paul J Hergenrother
Sep 22, 2012·Annual Review of Microbiology·Kenn Gerdes, Etienne Maisonneuve
Jun 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rick A Fasani, Michael A Savageau

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Datasets Mentioned

BETA
AAC00-1
KT149413
KT149414

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MUSCLE
MEGA
Oligo Analyzer
JMP
control
Bioedit

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