The vascularized periosteum flap as novel tissue engineering model for repair of cartilage defects

Journal of Cellular and Molecular Medicine
Leila HarhausChao-Min Cheng

Abstract

Periosteum is a promising tissue engineering scaffold in research of cartilage repair; so far however, periosteum transfers have not been realized successfully because of insufficient nourishment of the graft. In a translational approach we, for the first time, designed a vascularized periosteum flap as 'independent' biomaterial with its own blood supply to address this problem and to reconstruct circumscript cartilage defects. In six 3-month-old New Zealand rabbits, a critical size cartilage defect of the medial femur condyle was created and covered by a vascularized periosteum flap pedicled on the saphenous vessels. After 28 days, formation of newly built cartilage was assessed macroscopically, histologically and qualitatively via biomechanical compression testing, as well as on molecular biological level via immunohistochemistry. All wounds healed completely, all joints were stable and had full range of motion. All flaps survived and were perfused through their pulsating pedicles. They showed a stable attachment to the bone, although partially incomplete adherence. Hyaline cartilage with typical columnar cell distribution and positive Collagen II staining was formed in the transferred flaps. Biomechanical testing revealed a ...Continue Reading

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Citations

May 21, 2020·Scientific Reports·Hui-Yi HsiaoJung-Ju Huang
Feb 9, 2017·Stem Cells Translational Medicine·Nicole Y C YuMelissa L Knothe Tate
Dec 16, 2016·BoneKEy Reports·Melissa L Knothe TateUlf R Knothe

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