The vasodilator-stimulated phosphoprotein is regulated by cyclic GMP-dependent protein kinase during neutrophil spreading

The Journal of Immunology : Official Journal of the American Association of Immunologists
D W Lawrence, K B Pryzwansky

Abstract

The expression and phosphorylation state of the vasodilator-stimulated phosphoprotein (VASP), a membrane-associated focal adhesion protein, was investigated in human neutrophils. Adhesion and spreading of neutrophils induced the rapid phosphorylation of VASP. The phosphorylation of VASP was dependent on cell spreading, as VASP was expressed as a dephosphorylated protein in round adherent cells and was phosphorylated at the onset of changes in cell shape from round to spread cells. Immunofluorescence microscopy demonstrated that VASP was localized at the cell cortex in round cells and redistributed to focal adhesions at the ventral surface of the cell body during cell spreading. Dual labeling of spread cells indicated that VASP was colocalized with F-actin in filopodia and in focal adhesions, suggesting that the phosphorylation of VASP during cell spreading may be involved in focal adhesion complex organization and actin dynamics. VASP is a prominent substrate for both cGMP-dependent protein kinase (cGK) and cAMP-dependent protein kinase. Evidence suggested that cGK regulated neutrophil spreading, as both VASP phosphorylation and neutrophil spreading were inhibited by Rp-8-pCPT-cGMPS (cGK inhibitor), but not KT5720 (cAMP-depende...Continue Reading

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Citations

Jul 5, 2005·Circulation Research·Irina A KolosovaAlexander D Verin
Feb 4, 2009·Journal of Neurochemistry·François Paquet-DurandPer Ekström
Apr 12, 2014·Journal of the American Heart Association·Jochen SteppanLakshmi Santhanam
May 23, 2014·Cellular and Molecular Life Sciences : CMLS·Yulia ArtemenkoPeter N Devreotes
Apr 8, 2009·Infection and Immunity·Sarah E SzarowiczFrederick S Southwick
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudia G WernerKatherine B Pryzwansky
Jul 15, 2015·Metallomics : Integrated Biometal Science·Rui LiY James Kang
Mar 13, 2021·Drug Delivery and Translational Research·Aditya JosyulaLaura M Ensign
Aug 9, 2007·Journal of Cell Science·Susan L LindsayThomas J Evans
Oct 15, 2009·Journal of Cell Science·Peter M BenzThomas Renné
Sep 25, 2002·Biochemical and Biophysical Research Communications·Mauro BrunettiVirgilio Evangelista
Nov 13, 2003·Peptides·Ronald D MathisonEssam Metwally
Oct 19, 2006·Biochimica Et Biophysica Acta·Małgorzata CiumanWojciech A Gorczyca
Jun 5, 2008·Science Signaling·Erin C Rericha, Carole A Parent

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