The VC1777-VC1779 proteins are members of a sialic acid-specific subfamily of TRAP transporters (SiaPQM) and constitute the sole route of sialic acid uptake in the human pathogen Vibrio cholerae

Microbiology
Nityananda ChowdhuryE Fidelma Boyd

Abstract

Sialic acids are nine-carbon amino sugars that are present on all mucous membranes and are often used by bacteria as nutrients. In pathogenic Vibrio the genes for sialic acid catabolism (SAC) are known to be important for host colonization, yet the route for sialic acid uptake is not proven. Vibrio cholerae contains a tripartite ATP-independent periplasmic (TRAP) transporter, SiaPQM (VC1777-VC1779), encoded by genes within the Vibrio pathogenicity island-2 (VPI-2), which are adjacent to the SAC genes nanA, nanE and nanK. We demonstrate a correlation of the occurrence of VPI-2 and the ability of Vibrio to grow on the common sialic acid N-acetylneuraminic acid (Neu5Ac), and that a V. cholerae N16961 mutant defective in vc1777, encoding the large membrane protein component of the TRAP transporter, SiaM, is unable to grow on Neu5Ac as the sole carbon source. Using the genome context and known structures of the SiaP protein component of the TRAP transporter, we define a subfamily of Neu5Ac-specific TRAP transporters, of which the vc1777-vc1779 genes are the only representatives in V. cholerae. A recent report has suggested that an entirely different TRAP transporter (VC1927-VC1929) is the Neu5Ac transporter in V. cholerae. Bioinform...Continue Reading

References

Jun 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·S NeesF Mayer
Feb 1, 1992·Glycobiology·A Varki
Feb 1, 1992·Infection and Immunity·J E GalenJ B Kaper
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R K TaylorJ J Mekalanos
Jun 28, 1996·Science·M K Waldor, J J Mekalanos
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·D K KaraolisP R Reeves
Nov 12, 1998·Trends in Biochemical Sciences·F JeanmouginT J Gibson
Jun 29, 2002·Trends in Microbiology·Eric Vimr, Carol Lichtensteiger
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Eric R VimrSusan M Steenbergen
Apr 28, 2004·Plasmid·Nadège PhilippeDominique Schneider
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dong-Eun ChangTyrrell Conway
Feb 26, 2005·Infection and Immunity·Susan M SteenbergenEric R Vimr
Aug 23, 2005·Infection and Immunity·Simon AllenMichael A Apicella
Sep 5, 2007·Microbiology·Emmanuele SeveriGavin H Thomas
Dec 8, 2007·The Journal of Biological Chemistry·Emmanuele SeveriGavin H Thomas
Jul 9, 2008·Trends in Molecular Medicine·Ajit Varki
Jan 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher MulliganGavin H Thomas
May 28, 2009·BMC Evolutionary Biology·Salvador Almagro-Moreno, E Fidelma Boyd
Jul 1, 2009·Infection and Immunity·Salvador Almagro-Moreno, E Fidelma Boyd
Jun 30, 2010·FEMS Microbiology Reviews·Christopher MulliganGavin H Thomas
Jul 27, 2010·Trends in Microbiology·Marcus FischerGavin H Thomas
Jul 5, 2011·Journal of Bacteriology·Martina ValentiniKarine Lapouge
Oct 8, 2011·Microbiology·Gavin H Thomas, E Fidelma Boyd

❮ Previous
Next ❯

Citations

Jan 12, 2013·Microbial Ecology·Crystal N Johnson
Sep 28, 2012·MBio·Ryan W BogardJohn J Mekalanos
Jul 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·Thanuja Gangi SettyS Ramaswamy
Aug 29, 2012·FEMS Microbiology Letters·Nadine GruteserGavin H Thomas
May 13, 2014·Applied and Environmental Microbiology·Muireann EganDouwe van Sinderen
Jun 11, 2016·Biochemical Society Transactions·Gavin H Thomas
Sep 10, 2015·Microbiology Spectrum·Brandy L Haines-MengesE Fidelma Boyd
Nov 7, 2015·Microbiology Spectrum·E Fidelma BoydW Brian Whitaker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.