The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations.

Malaria Research and Treatment
Chris BassLinda M Field

Abstract

Regular monitoring of mosquito vector populations is an integral component of most vector control programmes. Contemporary data on mosquito species composition, infection status, and resistance to insecticides are a prerequisite for effective intervention. For this purpose we, with funding from the Innovative Vector Control Consortium (IVCC), have developed a suite of high-throughput assays based on a single "closed-tube" platform that collectively comprise the "Vector Population Monitoring Tool" (VPMT). The VPMT can be used to screen mosquito disease vector populations for a number of traits including Anopheles gambiae s.l. and Anopheles funestus species identification, detection of infection with Plasmodium parasites, and identification of insecticide resistance mechanisms. In this paper we focus on the Anopheles-specific assays that comprise the VPMT and include details of a new assay for resistance todieldrin Rdl detection. The application of these tools, general and specific guidelines on their use based on field testing in Africa, and plans for further development are discussed.

References

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Citations

May 16, 2014·PloS One·Muhammad AshfaqM Sajjad Mirza
Feb 27, 2013·The American Journal of Tropical Medicine and Hygiene·Michael R ReddyMichel A Slotman
Feb 7, 2018·International Journal of Environmental Research and Public Health·Konstantinos MitsakakisKonstantinos Mavridis
Nov 8, 2018·BioMed Research International·Behi K FodjoMouhamadou S Chouaïbou
Apr 26, 2019·Wellcome Open Research·Chouaïbou Seïdou MouhamadouBenjamin Guibehi Koudou
Mar 21, 2017·Wellcome Open Research·Mouhamadou ChouaïbouLuc Djogbenou

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Methods Mentioned

BETA
PCR
Enzyme-linked Immunosorbent Assay
genotyping
dissection
biosensors
biosensor
chips

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