The velvet protein VeA functions in asexual cycle, stress tolerance and transcriptional regulation of Beauveria bassiana

Fungal Genetics and Biology : FG & B
Ding-Yi WangMing-Guang Feng

Abstract

VeA is a key velvet protein that regulates sexual/asexual development and secondary metabolism in filamentous fungi, particularly Aspergilli, but has not been explored yet in asexual insect mycopathogens, such as Beauveria bassiana. Here, we report a localization of B. bassiana VeA in the cytoplasm of hyphal cells exposed to either light or dark cue and its migration to the nucleus only in darkness. Deletion of veA resulted in facilitated hyphal growth and decreased cell length on rich media, light growth defects on scant media, and increased sensitivities to oxidation, high osmolarity and prolonged heat shock during colony growth. Compared to wild-type, the deletion mutant was much more triggered in conidiation at optimal 25 °C in darkness than in a light/dark (L:D) cycle of 12:12, indicating the role of VeA acting as a negative regulator of conidiation in a light-dependent manner. The mutant conidia produced at L:D 12:12 showed defects in germination, thermotolerance and UVB resistance but no change in virulence, contrasting to attenuated virulence for the mutant conidia produced in darkness. Intriguingly, fungal outgrowth and conidiation were markedly suppressed on the surfaces of the mutant-mummified insect cadavers, sugges...Continue Reading

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